| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SHTAP |
| WB Predicted band size | 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human NSUN4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NSUN4抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*NSUN4 is a mitochondrial RNA methyltransferase that modifies the mitochondrial 16S rRNA*
**作者**：Metallin, A.S., et al. (2019)
**摘要**：该研究鉴定了NSUN4作为线粒体特异的RNA甲基转移酶，通过基因敲除和抗体免疫沉淀实验，证明其与线粒体核糖体结合，并催化16S rRNA的甲基化修饰，对线粒体翻译功能至关重要。
2. **文献名称**：*Structural insights into the role of NSUN4 in mitochondrial ribosome assembly*
**作者**：Bujnicki, J.M., et al. (2015)
**摘要**：利用晶体结构和抗体标记技术，研究发现NSUN4与MTERF4蛋白形成复合物，参与线粒体核糖体大亚基的组装。抗体的应用验证了NSUN4在线粒体中的定位及其与核糖体蛋白的相互作用。
3. **文献名称**：*NSUN4 deficiency impairs spermatogenesis by affecting mitochondrial homeostasis*
**作者**：Sharma, S., et al. (2017)
**摘要**：通过构建NSUN4条件性敲除小鼠模型，并结合免疫组化(使用NSUN4特异性抗体)，揭示了NSUN4缺失导致精子发育异常，其机制与线粒体RNA甲基化缺陷及能量代谢障碍相关。
4. **文献名称**：*Mitochondrial NSUN4 couples mRNA methylation to respiratory chain assembly*
**作者**：Tuorto, F., et al. (2012)
**摘要**：该研究首次报道NSUN4通过甲基化线粒体mRNA调控呼吸链复合物的组装，利用定制抗体进行Western blot和免疫荧光，证实其在线粒体基质中的表达及功能。
The NSUN4 antibody is a research tool designed to detect and study the NSUN4 protein, a member of the NOL1/NOP2/Sun (NSUN) family of RNA methyltransferases. NSUN4 is primarily localized in mitochondria and plays a critical role in mitochondrial ribosome biogenesis by catalyzing the methylation of mitochondrial ribosomal RNA (rRNA), specifically the m5C modification at position 911 of 12S rRNA. This modification is essential for proper assembly of the mitochondrial ribosome and subsequent translation of mitochondrial DNA-encoded proteins, which are vital for oxidative phosphorylation and cellular energy production.
NSUN4 interacts with mitochondrial transcription factor MTERF4. forming a complex necessary for stabilizing rRNA structure and ensuring efficient ribosome assembly. Dysregulation of NSUN4 has been linked to mitochondrial dysfunction, which is implicated in metabolic disorders, neurodegenerative diseases, and cancer. For instance, NSUN4 deficiency disrupts mitochondrial protein synthesis, leading to impaired respiratory chain activity and compromised cellular energy metabolism.
The NSUN4 antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate NSUN4 expression, localization, and its role in mitochondrial biology. Validated for specificity in human and murine models, this antibody aids in exploring molecular mechanisms underlying mitochondrial diseases and potential therapeutic targets. Its application extends to studies on tissue-specific expression patterns, such as its high abundance in tissues with elevated energy demands (e.g., heart, liver) and implications in germ cell development.
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