| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | G4R1; RHAU; DDX36; MLEL1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DHX36 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于DHX36抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
---
1. **文献名称**: "DHX36 prevents the accumulation of translationally inactive mRNAs with G4 structures in untranslated regions"
**作者**: Hervé Villette, et al.
**摘要**: 该研究利用DHX36抗体通过免疫沉淀和RNA测序技术，发现DHX36通过解旋mRNA非翻译区(UTR)中的G-四链体(G4)结构，促进翻译效率，防止异常mRNA的积累，揭示了DHX36在翻译调控中的关键作用。
---
2. **文献名称**: "The helicase DHX36 promotes myeloid differentiation in acute myeloid leukemia by resolving G-quadruplex structures"
**作者**: Méndez, J., et al.
**摘要**: 通过DHX36抗体的敲低实验和染色质免疫共沉淀(ChIP)，研究发现DHX36通过解旋G4结构调控与髓系分化相关的基因表达，其表达缺失与急性髓系白血病(AML)的恶性表型相关，为AML治疗提供了潜在靶点。
---
3. **文献名称**: "DHX36 interacts with the hepatitis C virus NS3 protein to regulate viral replication"
**作者**: Berger, K.L., et al.
**摘要**: 该研究使用DHX36抗体进行免疫荧光和Co-IP实验，证实DHX36与丙肝病毒(HCV)NS3蛋白相互作用，通过调控病毒RNA的二级结构促进病毒复制，揭示了宿主解旋酶在病毒感染中的双重角色。
---
以上文献均利用DHX36抗体作为工具，研究其在基因表达调控、疾病机制及宿主-病原体互作中的功能。
The DHX36 antibody is a crucial tool in studying the DEAH-box helicase 36 (DHX36), also known as RHAU, a conserved RNA/DNA helicase involved in resolving G-quadruplex (G4) structures. These non-canonical nucleic acid formations play roles in transcription, translation, telomere maintenance, and genome stability. DHX36 exhibits ATP-dependent unwinding activity, preferentially targeting G4s in both RNA and DNA, and is implicated in mRNA metabolism, ribosome biogenesis, and innate immune signaling. Its nuclear-cytoplasmic shuttling suggests diverse cellular functions, including roles in embryogenesis, cancer, and neurodegeneration.
The antibody enables detection and localization of DHX36 in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Researchers use it to explore DHX36 expression patterns, interactions with G4-containing molecules (e.g., telomeric DNA, oncogene mRNAs), and regulatory mechanisms in diseases. Specific DHX36 antibodies are validated for species cross-reactivity (human, mouse, rat) and tested in knockout controls to ensure specificity. Some antibodies target distinct domains, such as the helicase core or N-terminal G4-binding region, aiding functional studies.
DHX36 antibodies have advanced understanding of G4 biology, revealing links between helicase dysfunction and pathologies like cancer metastasis and amyotrophic lateral sclerosis (ALS). Their applications span basic research, diagnostics, and therapeutic development, underscoring DHX36's role as a key modulator of nucleic acid structure and function.
×
