| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/20000 | Human,Mouse,Rat |
| Aliases | POR |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human VDAC2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于VDAC2抗体的3篇参考文献及其简要摘要：
1. **《VDAC2 regulates BAX recruitment and activation in mitochondria-mediated apoptosis》**
- 作者：Cheng, E.H.Y. 等
- 摘要：该研究揭示VDAC2在线粒体凋亡途径中调控BAX蛋白的定位与激活，通过敲除实验及VDAC2特异性抗体证实其与BAX的相互作用，为癌症治疗提供新靶点。
2. **《Proteomic identification of mitochondrial VDAC2 interactomes reveals association with Hsp60》**
- 作者：Reina, S. 等
- 摘要：利用免疫共沉淀结合质谱技术，鉴定VDAC2的相互作用蛋白网络，发现其与Hsp60等分子伴侣结合，实验中通过VDAC2抗体验证蛋白互作机制。
3. **《Altered VDAC2 expression exacerbates cardiac dysfunction in diabetic mice》**
- 作者：Pecoraro, M. 等
- 摘要：研究糖尿病心肌病中线粒体VDAC2的表达变化，使用VDAC2抗体进行Western blot和免疫组化分析，发现VDAC2下调导致心肌细胞能量代谢障碍。
每篇文献均明确提及VDAC2抗体的应用，涵盖功能机制、蛋白互作及疾病模型研究。
**Background of VDAC2 Antibody**
The Voltage-Dependent Anion Channel 2 (VDAC2) is a mitochondrial outer membrane protein belonging to the VDAC family, which includes three isoforms (VDAC1. VDAC2. VDAC3). VDAC2 regulates the transport of ions, metabolites (e.g., ATP, ADP), and apoptotic factors across the mitochondrial membrane, playing a critical role in energy metabolism, calcium signaling, and apoptosis. Unlike VDAC1. VDAC2 exhibits unique functional roles, such as maintaining mitochondrial integrity, modulating reactive oxygen species (ROS) production, and serving as a platform for interactions with pro-apoptotic proteins like BAK.
VDAC2-specific antibodies are essential tools for studying its expression, localization, and interactions in various biological contexts. These antibodies enable detection and quantification of VDAC2 in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research involving VDAC2 antibodies has shed light on its involvement in diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions, where mitochondrial dysfunction is a hallmark. Additionally, VDAC2 antibodies aid in exploring its tissue-specific roles, post-translational modifications, and potential as a therapeutic target.
Validating VDAC2 antibody specificity is crucial due to high sequence homology among VDAC isoforms. Antibodies are often raised against unique peptide sequences or conformational epitopes to ensure isoform-selective recognition. Their applications extend to both basic research and clinical studies, emphasizing VDAC2's emerging significance in cellular homeostasis and pathology.
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