| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | STSL |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ABCG5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ABCG5抗体的3篇代表性文献，供参考：
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1. **文献名称**：*"Expression and localization of ABCG5 and ABCG8 in human liver, intestine, and kidney"*
**作者**：Lee MH, Lu K, Patel SB
**摘要**：该研究通过制备特异性ABCG5抗体，利用Western blot和免疫组化技术，揭示了ABCG5蛋白在小肠上皮细胞顶膜、肝细胞胆小管膜及肾近端小管中的定位，证实其在胆固醇和植物甾醇外排中的关键作用。
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2. **文献名称**：*"Mutations in ABCG5 or ABCG8 causing sitosterolemia alter ABCG5 protein stability"*
**作者**：Berge KE, Tian H, Graf GA et al.
**摘要**：研究利用ABCG5抗体分析谷甾醇血症患者突变体的蛋白表达，发现部分突变导致ABCG5稳定性下降，影响其与ABCG8的异二聚体形成，揭示了疾病相关的分子机制。
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3. **文献名称**：*"ABCG5/ABCG8-mediated cholesterol transport in macrophages modulates atherosclerosis progression"*
**作者**：Graf GA, Cohen JC, Hobbs HH
**摘要**：通过ABCG5抗体检测巨噬细胞中ABCG5/ABCG8复合体的表达，发现其通过促进胆固醇逆向转运延缓动脉粥样硬化斑块形成，为靶向治疗提供了实验依据。
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4. **文献名称**：*"Immunohistochemical analysis of ABCG5 in a mouse model of diet-induced obesity"*
**作者**：Calpe-Berdiel L, Rotllan N, Fievet C et al.
**摘要**：研究使用ABCG5抗体探究高脂饮食小鼠模型中ABCG5的肠肝表达变化，发现其表达下调与胆固醇代谢紊乱相关，提示其在肥胖相关代谢疾病中的潜在调控作用。
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以上文献涵盖ABCG5抗体的应用场景(蛋白检测、定位、功能研究及疾病机制)，均为领域内经典或高影响力研究。如需具体DOI或年份信息，可进一步补充检索。
ABCG5 antibodies are essential tools for studying the ATP-binding cassette subfamily G member 5 (ABCG5), a transmembrane protein critical for sterol homeostasis. ABCG5 forms a heterodimer with ABCG8. functioning as a cholesterol efflux transporter in the liver and intestine. This complex mediates the excretion of dietary sterols, including cholesterol and plant sterols, into bile and limits their absorption in the gut. Mutations in the ABCG5 gene are linked to sitosterolemia, a rare autosomal recessive disorder characterized by excessive accumulation of plant sterols in plasma and tissues, leading to cardiovascular and hematologic complications.
Researchers employ ABCG5 antibodies in various applications, such as Western blotting, immunohistochemistry, and immunofluorescence, to detect protein expression, localization, and regulation in tissues like the liver, intestine, and gallbladder. These antibodies aid in exploring ABCG5's role in lipid metabolism, drug resistance, and diseases like atherosclerosis or gallstone formation. Additionally, they are valuable in preclinical studies to evaluate therapeutic interventions targeting sterol transport pathways. High-specificity ABCG5 antibodies are crucial for distinguishing ABCG5 from related ABC transporters, ensuring accurate experimental outcomes. Their development and validation continue to support advancements in understanding sterol-related pathologies and potential treatments.
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