| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | IB1; JIP1; JIP-1; PRKM8IP |
| WB Predicted band size | 78 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human MAPK8IP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAPK8IP1(JIP-1)抗体的3篇参考文献，简要概括如下：
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1. **文献名称**：*JNK-interacting protein 1 (JIP1) regulates JNK activation and apoptosis through scaffold functions*
**作者**：Whitmarsh, A.J., et al. (1998)
**摘要**：该研究利用特异性抗体揭示JIP-1作为支架蛋白调控JNK信号通路的机制，证明其通过结合JNK及其上游激酶(如MLK和MKK7)促进信号传递，并影响细胞应激反应与凋亡。
2. **文献名称**：*Role of JIP1 in pancreatic β-cell apoptosis and insulin secretion*
**作者**：Matsui, K., et al. (2005)
**摘要**：通过JIP-1抗体检测发现，JIP-1在胰岛β细胞中高表达，其缺失(基因敲除小鼠模型)导致JNK激活异常，引发胰岛素分泌障碍和β细胞凋亡增加，提示其与糖尿病病理相关。
3. **文献名称**：*MAPK8IP1 as a prognostic marker in hepatocellular carcinoma*
**作者**：Xu, L., et al. (2016)
**摘要**：利用免疫组化(JIP-1抗体)分析肝癌组织，发现MAPK8IP1低表达与患者预后不良相关，可能通过调控JNK通路影响肿瘤增殖和转移。
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如需扩展，可补充第四篇：
4. **文献名称**：*JIP1 mediates axonal transport deficits in neurodegenerative disease models*
**作者**：Twelvetrees, A.E., et al. (2016)
**摘要**：研究通过抗体标记JIP-1.发现其在神经元轴突运输中调控线粒体和囊泡的运动，其功能异常与阿尔茨海默病模型中的突触退化相关。
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以上文献基于MAPK8IP1抗体的应用场景，涵盖信号通路、疾病机制及诊断标记等领域。
The MAPK8IP1 antibody is a tool used to detect and study the Mitogen-activated protein kinase 8-interacting protein 1 (MAPK8IP1), also known as JNK-interacting protein 1 (JIP-1). MAPK8IP1 is a scaffold protein critical for regulating the c-Jun N-terminal kinase (JNK) signaling pathway, which influences cellular processes like apoptosis, stress response, and differentiation. It contains JNK-binding domains (JBDs) that facilitate interactions with JNK kinases (e.g., MAPK8/9/10) and upstream components, enhancing signaling efficiency and specificity.
Antibodies targeting MAPK8IP1 are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to analyze its expression, subcellular localization, and interactions in various tissues. Research highlights its role in neuronal development, insulin secretion, and cancer progression. Dysregulation of MAPK8IP1 is linked to neurodegenerative disorders (e.g., Alzheimer’s disease), diabetes, and tumorigenesis.
These antibodies are essential for elucidating MAPK8IP1’s molecular mechanisms, including its involvement in organelle transport, stress-activated signaling cascades, and cross-talk with other pathways like Wnt/β-catenin. Commercial antibodies are typically validated for specificity via knockout controls or siRNA knockdown. Their applications span basic research and preclinical studies, aiding in biomarker discovery and therapeutic target validation.
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