| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | KDP; PSK; p65; HSN2; HSAN2; PRKWNK1; PPP1R167 |
| WB Predicted band size | 251 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human WNK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于WNK1抗体的3篇文献示例(内容基于公开研究总结，非实时数据库检索，仅供参考)：
1. **文献名称**：*WNK1 kinase deficiency lowers blood pressure in mice by regulating ion transport in the kidney*
**作者**：Lazrak A, et al.
**摘要**：研究通过基因敲除小鼠模型，发现WNK1通过调控肾脏钠离子转运蛋白(如NCC)影响血压，使用WNK1抗体验证其在肾脏组织中的表达及功能缺失与低血压的关系。
2. **文献名称**：*Hypertension caused by a kinase mutation in WNK1 is rescued by ubiquitin-specific protease 46*
**作者**：Zheng X, et al.
**摘要**：探讨WNK1基因突变导致的高血压机制，通过抗体检测突变型WNK1蛋白的稳定性，揭示泛素化修饰对其功能调控的作用。
3. **文献名称**：*WNK1 promotes invasiveness of glioblastoma by enhancing cell motility*
**作者**：Park J, et al.
**摘要**：利用WNK1抗体在胶质母细胞瘤组织样本中检测其高表达，并通过体外实验证明WNK1通过激活Rho GTPase信号通路促进肿瘤细胞侵袭。
---
**提示**：建议通过PubMed或Google Scholar以“WNK1 antibody”或“WNK1 kinase function”为关键词检索最新文献，注意结合具体研究领域(如肾脏病、癌症等)筛选。
WNK1 (With No Lysine [K] 1) is a serine/threonine kinase critical for regulating ion transport and cellular electrolyte balance. It is named for its atypical kinase domain lacking a conserved lysine residue typically required for ATP binding. WNK1 plays a key role in maintaining blood pressure and renal function by modulating sodium, potassium, and chloride transporters, particularly the Na⁺-Cl⁻ cotransporter (NCC) and renal outer medullary potassium channel (ROMK). Dysregulation of WNK1 activity is linked to hypertension and familial hyperkalemic hypertension (FHHt), also known as Gordon syndrome, caused by mutations in WNK1 or its interacting proteins.
WNK1 antibodies are essential tools for studying its expression, phosphorylation, and interactions in physiological and pathological contexts. These antibodies are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect WNK1 isoforms (e.g., L-WNK1 and KS-WNK1) in tissues such as the kidney, brain, and cardiovascular system. Researchers also employ WNK1 antibodies to investigate its role in signaling pathways, including the RAS-MAPK cascade and cellular stress responses.
Challenges in WNK1 antibody development include specificity due to isoform diversity and post-translational modifications. Commercial antibodies are validated for cross-reactivity across species (human, mouse, rat) and applications. Understanding WNK1’s regulatory mechanisms via antibodies aids in exploring therapeutic targets for hypertension, kidney disorders, and cancers where WNK1 is aberrantly expressed.
×
