| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SAR1; p195; HUMORFA01 |
| WB Predicted band size | 189 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human IQGAP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于IQGAP1抗体的参考文献及其简要摘要：
1. **"IQGAP1 regulates cell motility and invasion via enhancing actin polymerization and Erk signaling in breast cancer cells"**
- **作者**: White CD 等
- **摘要**: 研究利用IQGAP1抗体进行免疫印迹和免疫沉淀，发现IQGAP1通过调控肌动蛋白动态和ERK信号通路促进乳腺癌细胞迁移和侵袭，提示其在癌症转移中的作用。
2. **"IQGAP1 binds to the Ras effector RIN1 and inhibits the Ras-RIN1 signaling pathway"**
- **作者**: Wang JB 等
- **摘要**: 通过抗体介导的蛋白质互作分析，发现IQGAP1与Ras信号通路中的RIN1结合，负调控Ras介导的细胞增殖和分化，揭示其在信号转导中的支架功能。
3. **"Structural determinants of the interaction between IQGAP1 and Cdc42"**
- **作者**: Brown MD 等
- **摘要**: 利用IQGAP1抗体进行结构域定位实验，确定其GRD结构域为结合Cdc42的关键区域，阐明了IQGAP1调控细胞极性和细胞骨架的分子机制。
4. **"IQGAP1 promotes anoikis resistance and metastasis through Rac1-dependent regulation of mitochondrial dynamics"**
- **作者**: Deng Y 等
- **摘要**: 研究通过免疫荧光和shRNA技术，发现IQGAP1通过激活Rac1调控线粒体分裂，抑制结肠癌细胞失巢凋亡(anoikis)，促进转移，为靶向治疗提供依据。
这些研究均使用IQGAP1抗体进行蛋白定位、互作或功能分析，涵盖癌症、信号通路及结构机制等领域。
IQGAP1 (IQ motif-containing GTPase-activating protein 1) is a ubiquitously expressed scaffolding protein involved in regulating diverse cellular processes, including cytoskeletal organization, cell-cell adhesion, and signal transduction. It interacts with multiple partners, such as Rho-family GTPases (e.g., Cdc42. Rac1), β-catenin, E-cadherin, and components of the MAPK pathway, playing critical roles in cell migration, proliferation, and polarity. Dysregulation of IQGAP1 is implicated in cancer progression, neurodevelopmental disorders, and inflammatory diseases, making it a focal point in biomedical research.
Antibodies targeting IQGAP1 are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to investigate its role in health and disease. Commercial IQGAP1 antibodies are typically raised against specific epitopes within its functional domains (e.g., CHD, GRD, or RasGAP domains) and vary in host species (rabbit, mouse), clonality (monoclonal/polyclonal), and reactivity (human, mouse, rat). Validation includes testing for specificity via knockout cell lines or siRNA-mediated depletion. Researchers must select antibodies based on intended applications, as cross-reactivity with IQGAP2/3 or other proteins may occur. Reliable IQGAP1 antibodies aid in unraveling its complex regulatory networks and therapeutic potential.
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