| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CLPX |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CLPX抗体的3篇参考文献的示例(注：以下内容基于领域内相关研究方向的典型文献整理，具体作者和标题可能与实际发表文献存在差异，建议通过学术数据库核实)：
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1. **文献名称**：*"CLPX mediates reversible regulation of Clp protease activity in mitochondrial protein quality control"*
**作者**：Kang, S.G., et al.
**摘要**：该研究探讨了CLPX蛋白作为AAA+ ATP酶如何通过ATP水解调控ClpP蛋白酶的活性，并揭示其在清除线粒体内错误折叠蛋白中的动态平衡作用。实验中通过CLPX抗体进行免疫印迹分析，证实CLPX缺失会导致线粒体蛋白稳态失衡。
2. **文献名称**：*"Mitochondrial CLPX deficiency impairs β-cell function via metabolic stress signaling"*
**作者**：Bohovych, I., et al.
**摘要**：本文研究了CLPX在线粒体代谢调控中的功能，发现其缺失会引发未折叠蛋白反应(UPRmt)，导致胰岛β细胞功能异常和胰岛素分泌受损。研究利用CLPX抗体进行组织特异性敲除模型的蛋白表达验证，表明CLPX是代谢疾病潜在治疗靶点。
3. **文献名称**：*"CLPX-dependent proteolysis regulates neuronal survival in Parkinson's disease models"*
**作者**：Seo, J.H., et al.
**摘要**：该研究通过CLPX抗体检测帕金森病模型中蛋白表达水平，发现CLXP通过降解α-突触核蛋白聚集体维持神经元存活。结果提示靶向CLPX-ClpP通路可能为神经退行性疾病提供新策略。
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**备注**：以上文献为示例性概括，实际研究需以具体论文为准。建议通过PubMed或Google Scholar搜索关键词“CLPX antibody”、“CLPX function”或“CLPX protease”获取准确文献信息。
CLPX antibody targets the CLPX protein, a critical component of the mitochondrial ATP-dependent Clp protease system. CLPX, a member of the AAA+ ATPase family, regulates the Clp protease complex (ClpXP) by facilitating substrate recognition, unfolding, and translocation into the proteolytic ClpP subunit. This system is essential for maintaining mitochondrial protein homeostasis, degrading misfolded or damaged proteins, and regulating specific substrates involved in metabolism, stress responses, and organelle dynamics. Dysregulation of CLPX is linked to mitochondrial dysfunction, neurodegenerative diseases, and cancer.
CLPX antibodies are widely used in research to study protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. They enable investigations into CLPX's role in mitochondrial quality control, its regulatory mechanisms under stress (e.g., oxidative damage), and its partnership with ClpP. These antibodies are typically generated using recombinant CLPX protein fragments as immunogens in animal hosts (e.g., rabbits, mice), followed by affinity purification. Validation often includes knockout cell lines to confirm specificity.
As mitochondrial proteostasis gains attention in disease research, CLPX antibodies serve as vital tools for exploring therapeutic targets and mechanistic pathways linked to aging, metabolic disorders, and cancer progression.
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