| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | dgkd-2; DGKdelta |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human DGKD |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DGKD抗体的参考文献示例，内容基于典型研究场景整理：
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1. **文献名称**: *DGKD regulates diacylglycerol metabolism and T cell signaling in immune response*
**作者**: Sakane F, et al.
**摘要**: 本研究通过Western blot和免疫荧光技术，使用特异性DGKD抗体探究了DGKD在T细胞活化中的亚细胞定位及功能，发现其通过调控DAG代谢影响下游信号通路。
2. **文献名称**: *Role of DGKD in insulin resistance and hepatic lipid metabolism*
**作者**: Yasuda S, et al.
**摘要**: 作者利用DGKD抗体检测小鼠肝脏组织中DGKD的表达水平，证实其缺失导致脂质代谢异常和胰岛素敏感性下降，提示DGKD在代谢疾病中的潜在作用。
3. **文献名称**: *DGKD expression correlates with cancer progression in glioblastoma*
**作者**: Merida I, et al.
**摘要**: 通过免疫组化(IHC)结合DGKD抗体，研究发现DGKD在胶质母细胞瘤中高表达，且与患者生存率负相关，提示其作为肿瘤标志物的可能性。
4. **文献名称**: *Phosphorylation-dependent regulation of DGKD enzymatic activity*
**作者**: Shulga YV, et al.
**摘要**: 该研究采用免疫共沉淀(Co-IP)和激酶活性分析，利用DGKD抗体验证其磷酸化修饰对酶活性的调控机制，揭示了其在细胞信号动态平衡中的关键作用。
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**注**：以上文献信息为示例性整理，实际文献需通过学术数据库检索获取。建议使用PubMed或Web of Science以关键词“DGKD antibody”或“Diacylglycerol kinase delta”查找最新研究。
The diacylglycerol kinase delta (DGKD) antibody is a crucial tool for studying DGKD, a lipid-metabolizing enzyme belonging to the diacylglycerol kinase (DGK) family. DGKD catalyzes the conversion of diacylglycerol (DAG) to phosphatidic acid (PA), regulating lipid-mediated signaling pathways involved in cell proliferation, differentiation, and apoptosis. Specifically, DGKD modulates DAG-PA balance, which influences key processes like T-cell receptor signaling, neuronal development, and cancer progression. Dysregulation of DGKD has been linked to immune disorders, metabolic diseases, and malignancies.
The DGKD antibody enables researchers to detect and quantify DGKD expression in various tissues and cell types through techniques like Western blotting, immunohistochemistry, and immunofluorescence. It also aids in studying post-translational modifications, subcellular localization, and protein-protein interactions of DGKD. As DGKD is primarily localized to the cytoplasm and nucleus, the antibody helps elucidate its role in nuclear lipid signaling and transcriptional regulation.
Recent studies highlight DGKD's potential as a therapeutic target, particularly in cancers where altered lipid signaling drives tumor growth. The antibody's specificity and reliability are critical for validating DGKD's functional roles and exploring its diagnostic or prognostic value. Commercial DGKD antibodies are typically developed using immunogenic peptides or recombinant proteins, with validation across species like humans, mice, and rats, supporting cross-disciplinary research in immunology, oncology, and neuroscience.
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