| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AP-2; BOFS; AP2TF; TFAP2; AP-2alpha |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human TFAP2A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于TFAP2A抗体的代表性文献概览：
1. **"TFAP2A regulates cranial neural crest development"**
*作者：Zhang et al., 2020*
摘要：研究使用TFAP2A抗体在小鼠胚胎中进行免疫荧光染色，揭示TFAP2A在颅神经嵴细胞迁移和分化中的关键作用，证实其抗体特异性通过敲除模型验证。
2. **"Immunohistochemical analysis of TFAP2A in breast cancer subtypes"**
*作者：Williams et al., 2017*
摘要：通过免疫组化(使用TFAP2A单克隆抗体)分析乳腺癌组织样本，发现TFAP2A高表达与三阴性乳腺癌亚型显著相关，提示其作为潜在预后标志物。
3. **"ChIP-seq identifies TFAP2A target genes in keratinocytes"**
*作者：Roth et al., 2015*
摘要：采用TFAP2A抗体进行染色质免疫沉淀测序(ChIP-seq)，鉴定了角蛋白细胞中受TFAP2A调控的基因网络，揭示其在表皮屏障形成中的调控机制。
4. **"Validation of TFAP2A antibody specificity by CRISPR knockout"**
*作者：Li et al., 2019*
摘要：通过CRISPR/Cas9敲除TFAP2A基因，结合Western blot和流式细胞术验证商业抗体的特异性，为后续研究提供可靠的抗体选择依据。
(注：以上文献信息为模拟示例，实际引用需以具体论文为准。)
The TFAP2A antibody is a crucial tool in molecular biology research, targeting the Transcription Factor AP-2 Alpha (TFAP2A), a member of the AP-2 family of DNA-binding proteins. TFAP2A plays a pivotal role in embryonic development, particularly in craniofacial morphogenesis, neural crest cell differentiation, and limb formation. It functions as a sequence-specific transcriptional activator, regulating genes involved in cell proliferation, apoptosis, and tissue-specific differentiation by binding to GC-rich promoter regions. Dysregulation of TFAP2A has been implicated in various cancers, including breast cancer, melanoma, and neuroblastoma, where it can act as either a tumor suppressor or oncogene depending on cellular context.
The TFAP2A antibody is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study protein expression, localization, and function. Researchers employ it to investigate developmental disorders, such as branchiooculofacial syndrome linked to TFAP2A mutations, and to explore cancer biology through its interactions with pathways like p53 and EGFR. Validated antibodies specifically recognize conserved epitopes across species, enabling cross-species studies. However, interpretation requires careful validation due to potential isoform cross-reactivity. Its applications span developmental biology, oncology, and stem cell research, making it essential for understanding gene regulatory networks and disease mechanisms.
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