| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | p65; NFKB3 |
| WB Predicted band size | 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human RELA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RELA抗体的3篇示例文献(注：内容为模拟示例，实际文献需通过学术数据库查询)：
1. **文献名称**: "RELA/p65-specific antibody validation in NF-κB signaling studies"
**作者**: Chen, F., et al.
**摘要**: 该研究验证了多种RELA抗体的特异性，比较了它们在Western blot、免疫组化(IHC)和染色质免疫沉淀(ChIP)中的表现，为NF-κB信号通路研究提供了抗体选择指南。
2. **文献名称**: "Phosphorylation-dependent regulation of RELA activity in inflammation"
**作者**: Yamamoto, Y., & Gaynor, R.B.
**摘要**: 通过磷酸化特异性RELA抗体，揭示了RELA在炎症反应中的激活机制，发现其Ser536位点磷酸化对核转位和靶基因转录调控的关键作用。
3. **文献名称**: "RELA antibody-based detection of NF-κB activation in tumor microenvironments"
**作者**: Li, Q., & Verma, I.M.
**摘要**: 利用RELA抗体分析癌症组织中NF-κB的活化状态，发现肿瘤微环境中RELA核定位与化疗耐药性显著相关，为靶向治疗提供依据。
如需具体文献，建议在PubMed或Web of Science中检索关键词“RELA antibody validation”或“NF-κB p65 antibody application”。
The RELA antibody targets the RELA protein, also known as p65. a critical subunit of the NF-κB transcription factor family. NF-κB regulates genes involved in inflammation, immune response, cell proliferation, and apoptosis. RELA, in particular, is activated by diverse stimuli, including cytokines, pathogens, and stress signals, through the canonical NF-κB pathway. Upon activation, RELA is released from inhibitory IκB proteins, translocates to the nucleus, and drives the transcription of target genes.
RELA antibodies are widely used in research to study NF-κB signaling dynamics. They enable the detection of RELA expression, phosphorylation status, and subcellular localization via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Additionally, RELA-specific antibodies are employed in chromatin immunoprecipitation (ChIP) to identify DNA-binding sites, elucidating its role in gene regulation.
Dysregulation of RELA is linked to chronic inflammation, cancer, autoimmune diseases, and neurodegenerative disorders. Researchers use these antibodies to explore therapeutic strategies targeting NF-κB activity. Specificity and validation are critical, as cross-reactivity with other NF-κB subunits (e.g., RELB or c-REL) can compromise data accuracy. Overall, RELA antibodies are indispensable tools for unraveling NF-κB biology and its implications in health and disease.
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