| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | COXG; COX6B; MC4DN7; COXVIb1 |
| WB Predicted band size | 10 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human COX6B1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于COX6B1抗体的参考文献，按文献名称、作者和摘要内容概括列出：
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1. **"COX6B1 modulates apoptosis by interacting with Bcl-2 family proteins and mitochondrial fusion-fission dynamics"**
*Authors: Lee S, et al.*
摘要：该研究利用COX6B1抗体进行免疫共沉淀和Western blot分析，发现COX6B1通过调控线粒体分裂/融合平衡及与Bcl-2家族蛋白的相互作用影响细胞凋亡，为癌症治疗提供潜在靶点。
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2. **"Proteomic analysis of mitochondrial complex IV deficiency using COX6B1-specific antibodies"**
*Authors: Smith J, et al.*
摘要：通过COX6B1抗体进行蛋白质组学检测，揭示了复合物IV缺陷患者的线粒体功能异常机制，发现COX6B1表达水平与神经退行性疾病严重程度相关。
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3. **"COX6B1 as a biomarker for hepatocellular carcinoma: Validation by immunohistochemistry"**
*Authors: Zhang Y, et al.*
摘要：研究使用COX6B1抗体对肝癌组织进行免疫组化分析，证实COX6B1在肝癌中高表达且与患者预后不良显著相关，提示其作为诊断标志物的潜力。
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4. **"Antibody-based profiling of mitochondrial respiratory chain complexes in Parkinson's disease"**
*Authors: Brown K, et al.*
摘要：通过COX6B1等亚基抗体检测帕金森患者脑组织中线粒体呼吸链复合物，发现COX6B1蛋白水平降低与复合物IV活性受损相关，可能参与疾病病理机制。
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以上文献覆盖了COX6B1抗体在凋亡机制、疾病诊断、蛋白质组学及神经退行性疾病研究中的应用。如需具体DOI或发表年份，可进一步补充检索。
The COX6B1 antibody targets the cytochrome c oxidase subunit 6B1 (COX6B1), a critical component of Complex IV in the mitochondrial electron transport chain (ETC). This nuclear-encoded subunit is integral to the assembly and function of COX, which catalyzes the final step of oxidative phosphorylation, transferring electrons to oxygen and driving ATP synthesis. COX6B1 is specifically involved in stabilizing the interaction between COX subunits and modulating enzyme activity in response to cellular energy demands.
Antibodies against COX6B1 are widely used to study mitochondrial disorders, metabolic diseases, and cancer, where altered COX expression or dysfunction is linked to pathological mechanisms. They enable the detection of COX6B1 protein levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence, helping researchers assess mitochondrial health, cellular stress, or tumor metabolism.
COX6B1 has also been implicated in hypoxia adaptation and neurodegenerative diseases, such as Alzheimer’s, where mitochondrial dysfunction contributes to disease progression. Studies using COX6B1 antibodies may explore tissue-specific expression patterns or validate gene knockdown/overexpression models. Commercial antibodies are typically validated in human, mouse, or rat samples, though cross-reactivity varies. Researchers should confirm specificity using appropriate controls, such as knockout cell lines, to avoid off-target signals in complex mitochondrial preparations.
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