| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | IKBA; MAD-3; NFKBI |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NFKBIA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NFKBIA抗体的3篇参考文献示例(内容为模拟概括，建议通过学术数据库核实具体文献)：
1. **"Characterization of NFKBIA Antibody Specificity in Inflammatory Signaling"**
- 作者：Smith J et al.
- 摘要：研究验证了一种新型NFKBIA抗体的特异性，通过Western blot和免疫荧光证实其在检测人细胞中内源性IκBα(NFKBIA蛋白)的表达及降解动态中的应用，适用于NF-κB通路激活研究。
2. **"NFKBIA Antibody Reveals Altered Expression in Colorectal Cancer Tissues"**
- 作者：Li X et al.
- 摘要：利用NFKBIA抗体进行免疫组化分析，发现结直肠癌组织中IκBα表达显著下调，提示其可能作为肿瘤进展的生物标志物，并与患者预后相关。
3. **"Development of a Monoclonal Antibody Targeting Phosphorylated NFKBIA for Autoimmune Disease Studies"**
- 作者：Wang H et al.
- 摘要：报道了一种靶向NFKBIA磷酸化位点(Ser32/Ser36)的单克隆抗体制备，成功应用于类风湿性关节炎模型小鼠的炎症机制研究，揭示磷酸化IκBα在疾病中的调控作用。
如需具体文献，建议通过PubMed或Google Scholar检索关键词“NFKBIA antibody application”或“IκBα antibody study”获取最新研究。
The NFKBIA antibody is a crucial tool for studying the regulation of the NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathway. NFKBIA encodes IκBα (inhibitor of nuclear factor kappa B alpha), a key regulatory protein that binds to NF-κB transcription factors, sequestering them in the cytoplasm and preventing their nuclear translocation. Under basal conditions, IκBα maintains NF-κB in an inactive state. Upon stimulation by pro-inflammatory cytokines, pathogens, or stress signals, the IκB kinase (IKK) complex phosphorylates IκBα, marking it for ubiquitination and proteasomal degradation. This releases NF-κB, allowing it to enter the nucleus and activate genes involved in immune responses, inflammation, cell survival, and proliferation.
Antibodies targeting NFKBIA/IκBα are widely used in research to detect protein expression, phosphorylation status (e.g., at Ser32/Ser36), and degradation dynamics. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence to study NF-κB pathway activation in diseases such as cancer, autoimmune disorders, and chronic inflammation. Dysregulation of IκBα, through mutations or altered expression, is linked to NF-κB hyperactivation, contributing to tumor progression and therapy resistance. Researchers also use these antibodies to assess the effects of therapeutic agents targeting the NF-κB pathway. Specificity for different isoforms or post-translationally modified forms of IκBα ensures precise experimental insights. Proper sample handling (e.g., protease/phosphatase inhibitors) is critical due to the rapid degradation of IκBα upon cellular stimulation.
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