| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | COX11P |
| WB Predicted band size | 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human COX11 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于COX11抗体的模拟参考文献示例(注：部分文献为假设性描述，供参考格式)：
1. **文献名称**: *COX11 Antibody Characterization in Mitochondrial Complex IV Assembly*
**作者**: Smith J, et al.
**摘要**: 该研究开发了一种高特异性COX11多克隆抗体，用于Western blot和免疫荧光实验，验证了COX11在线粒体膜中的定位及其在细胞色素c氧化酶(复合体IV)组装中的关键作用。
2. **文献名称**: *COX11 Deficiency and Cardiac Hypertrophy: A Role in Copper Homeostasis*
**作者**: Tanaka M, et al.
**摘要**: 通过COX11抗体检测，研究发现COX11敲除小鼠心脏组织中铜离子稳态紊乱，导致复合体IV功能异常和心肌肥厚，揭示了COX11在铜伴侣机制中的重要性。
3. **文献名称**: *Immunohistochemical Analysis of COX11 in Neurodegenerative Disorders*
**作者**: Lee S, et al.
**摘要**: 利用COX11抗体对阿尔茨海默病脑组织进行免疫组化分析，发现COX11表达水平与线粒体功能障碍程度相关，提示其作为神经退行性病变的生物标志物潜力。
4. **文献名称**: *COX11 Interacts with SCO1/2: Antibody-Based Co-IP Studies*
**作者**: Garcia J, et al.
**摘要**: 通过COX11抗体进行免疫共沉淀实验，证实COX11与SCO1/2蛋白的相互作用，阐明其在铜离子转运至复合体IV活性中心的功能协同机制。
**备注**：以上文献为示例性质，实际引用时建议通过PubMed或Google Scholar以关键词“COX11 antibody”检索最新研究。
The COX11 antibody is a crucial tool in studying the cytochrome c oxidase (COX) complex, a key component of the mitochondrial electron transport chain. COX11. a nuclear-encoded protein, plays an essential role in the assembly of mitochondrial respiratory Complex IV (COX), specifically facilitating copper ion insertion into the catalytic subunit COX1. This process is vital for cellular energy production via oxidative phosphorylation. Dysregulation of COX11 or its associated pathways has been linked to mitochondrial disorders, neurodegenerative diseases, and cancer.
COX11 antibodies are widely used in research to investigate protein expression, localization, and interactions in mitochondrial studies. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess COX11 levels in tissues or cultured cells, particularly in contexts of mitochondrial dysfunction. Additionally, these antibodies aid in exploring COX11's role in diseases such as Leigh syndrome, cardiomyopathy, and tumors, where altered mitochondrial metabolism is implicated.
Most commercially available COX11 antibodies are raised in rabbits or mice using specific peptide antigens, with validation across species like human, mouse, and rat. Researchers prioritize antibodies validated for specificity through knockout controls or siRNA silencing. Recent studies also utilize COX11 antibodies to probe its interactions with copper chaperones and redox-sensitive pathways, expanding understanding of mitochondrial metal homeostasis and stress responses.
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