| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | DSI,GIT,PDI,PHDB,PDIA1,PO4DB,PO4HB,PROHB,CLCRP1,ERBA2L,P4Hbeta |
| Entrez GeneID | 5034 |
| clone | 4E10C6 |
| WB Predicted band size | 57.1kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human P4HB (AA: 309-508) expressed in Mammalian. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CYP1A1抗体的3篇参考文献示例(内容基于领域内常见研究方向，仅供参考)：
1. **文献名称**：*"Immunohistochemical analysis of CYP1A1 expression in human lung tissue: association with smoking and PAH exposure"*
**作者**：Smith, J.R. et al.
**摘要**：通过免疫组化技术，使用CYP1A1抗体检测吸烟者和非吸烟者肺组织中CYP1A1蛋白的表达水平，发现吸烟者CYP1A1表达显著升高，提示其可能参与多环芳烃(PAH)的代谢激活。
2. **文献名称**：*"Role of CYP1A1 in breast cancer: Antibody-based quantification and correlation with estrogen metabolism"*
**作者**：Lee, H. & Gonzalez, F.J.
**摘要**：利用Western blot和免疫荧光技术分析乳腺癌细胞中CYP1A1蛋白的表达，发现CYP1A1高表达与雌激素代谢物积累相关，可能影响肿瘤进展。
3. **文献名称**：*"Development of a monoclonal antibody specific to human CYP1A1 for toxicological studies"*
**作者**：Yamazaki, Y. et al.
**摘要**：报道了一种高特异性CYP1A1单克隆抗体的开发，验证其在肝细胞和体外模型中检测CYP1A1蛋白的可靠性，适用于环境毒素代谢研究。
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**注**：以上文献为示例性内容，实际引用时请通过PubMed或Web of Science等平台检索真实文献(可尝试关键词：CYP1A1 antibody, immunohistochemistry, polymorphism, cancer)。
The cytochrome P450 1A1 (CYP1A1) antibody is a crucial tool for studying the expression and function of the CYP1A1 enzyme, a member of the cytochrome P450 superfamily involved in xenobiotic metabolism. CYP1A1 plays a key role in metabolizing environmental pollutants, such as polycyclic aromatic hydrocarbons (PAHs), and certain drugs, often converting them into reactive intermediates. Its expression is tightly regulated by the aryl hydrocarbon receptor (AhR) pathway, which is activated upon exposure to ligands like dioxins. Researchers use CYP1A1 antibodies in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein levels in tissues or cell lines, providing insights into metabolic activity, toxicology, and disease mechanisms.
Elevated CYP1A1 expression has been linked to chemical toxicity, cancer progression (e.g., lung, breast), and metabolic disorders, making it a biomarker for environmental exposure or pathological states. The antibody’s specificity helps distinguish CYP1A1 from related isoforms (e.g., CYP1A2) and assess interindividual variability in drug responses. Cross-reactivity with homologs in common model organisms (e.g., rodents) further supports translational research. However, variability in antibody performance (epitope recognition, species compatibility) requires careful validation. Overall, CYP1A1 antibodies are vital for unraveling its role in detoxification, carcinogenesis, and personalized medicine.
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