| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ET3; ET-3; WS4B; HSCR4; PPET3 |
| WB Predicted band size | 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human EDN3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于EDN3抗体的虚构参考文献示例(仅供格式参考，具体内容需查阅真实数据库)：
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1. **标题**: *EDN3 Antibody-Based Detection of Endothelin-3 in Melanoma Progression*
**作者**: A. Smith et al.
**摘要**: 本研究利用特异性EDN3单克隆抗体，通过免疫组化技术分析黑色素瘤组织中EDN3的表达水平，发现EDN3高表达与肿瘤转移和患者预后不良显著相关，提示其作为潜在生物标志物的价值。
2. **标题**: *Role of EDN3 in Enteric Nervous System Development: Insights from Antibody Blockade Experiments*
**作者**: B. Lee & C. Garcia
**摘要**: 通过EDN3中和抗体阻断斑马鱼胚胎模型中的EDN3信号通路，研究发现EDN3缺失导致肠神经嵴细胞迁移异常，证实EDN3对肠神经系统发育的关键调控作用。
3. **标题**: *EDN3 Antibody Validation for Immunoblotting in Colorectal Cancer Studies*
**作者**: M. Tanaka et al.
**摘要**: 本文系统验证了商业化EDN3多克隆抗体的特异性，通过Western blot和siRNA敲低实验证明其可准确检测结直肠癌细胞系中EDN3蛋白表达，为后续机制研究提供可靠工具。
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**提示**：以上为模拟内容，实际文献请通过PubMed、Google Scholar等平台检索关键词“EDN3 antibody”或“Endothelin-3 antibody”获取。
**Background of EDN3 Antibody**
Endothelin-3 (EDN3) is a 21-amino acid peptide belonging to the endothelin family, which includes EDN1. EDN2. and EDN3. These peptides bind to G protein-coupled receptors (EDNRA and EDNRB) to regulate diverse physiological processes, including vasoconstriction, cell proliferation, and neural crest cell development. EDN3 plays a critical role in the enteric nervous system (ENS) formation, melanocyte development, and cardiovascular function. Dysregulation of EDN3 signaling is linked to congenital disorders like Hirschsprung’s disease (characterized by aganglionic megacolon) and Waardenburg syndrome, as well as cancers such as melanoma and neuroendocrine tumors.
The EDN3 antibody is a targeted immunoassay tool designed to detect and quantify EDN3 protein expression in tissues or biological samples. It is commonly used in research to study EDN3’s spatial and temporal expression patterns, its interaction with receptors, and its involvement in developmental or pathological contexts. Polyclonal or monoclonal EDN3 antibodies are validated for techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). By enabling precise visualization of EDN3. these antibodies contribute to understanding its role in diseases and evaluating potential therapeutic strategies targeting the endothelin pathway.
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