| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1 |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human HDAC8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HDAC8抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Histone deacetylase HDAC8 associates with smooth muscle α-actin and is essential for smooth muscle cell contractility*
**作者**：Waltregny, D., et al.
**摘要**：该研究揭示了HDAC8在平滑肌细胞中的功能，发现其通过调控α-肌动蛋白的乙酰化水平影响细胞收缩性，并利用特异性抗体证实HDAC8在癌症相关平滑肌异常中的潜在作用。
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2. **文献名称**：*Structural basis for the inhibition of histone deacetylase 8 by isoform-specific inhibitors*
**作者**：Olson, D.E., et al.
**摘要**：通过X射线晶体学和抗体验证，研究解析了HDAC8的三维结构及其与抑制剂的相互作用，为开发针对HDAC8的癌症治疗药物提供了分子基础。
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3. **文献名称**：*HDAC8 mutations in Cornelia de Lange syndrome affect the cohesin acetylation cycle*
**作者**：Deardorff, M.A., et al.
**摘要**：该研究利用HDAC8抗体检测患者细胞中的蛋白表达，发现HDAC8突变破坏染色质结构蛋白的乙酰化平衡，导致Cornelia de Lange综合征的神经发育缺陷。
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这些文献涵盖了HDAC8在细胞功能、结构机制及疾病中的研究，抗体应用包括蛋白定位、功能验证及临床关联分析。
Histone deacetylase 8 (HDAC8) is a class I zinc-dependent deacetylase involved in epigenetic regulation by removing acetyl groups from lysine residues on histones and non-histone proteins. It localizes to both the nucleus and cytoplasm, distinguishing it from other class I HDACs (e.g., HDAC1-3) that are primarily nuclear. HDAC8 plays critical roles in cell cycle progression, transcriptional regulation, and chromatin remodeling, with emerging links to developmental processes, metabolic pathways, and immune responses. Dysregulation of HDAC8 is implicated in cancers (e.g., neuroblastoma, leukemia), Cornelia de Lange syndrome (CdLS, linked to mutations in HDAC8), and neurological disorders.
HDAC8-specific antibodies are essential tools for studying its expression, localization, and functional interactions. These antibodies enable detection via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Due to structural similarities among HDAC isoforms, antibody specificity is critical to avoid cross-reactivity. Validated HDAC8 antibodies help investigate its role as a therapeutic target, particularly in evaluating HDAC inhibitors (e.g., selective HDAC8 inhibitors in preclinical cancer studies). Recent research also explores HDAC8's non-canonical substrates, expanding its biological relevance beyond epigenetic regulation.
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