| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60 |
| WB Predicted band size | 61 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human HSPD1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HSPD1抗体的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*Heat Shock Protein 60 in Immune Regulation and Cancer*
**作者**：Cohen-Sfady et al.
**摘要**：研究探讨了HSP60(HSPD1)在肿瘤微环境中的免疫调节作用，通过特异性抗体检测发现HSP60在癌细胞表面异常表达，可能通过调控T细胞活性促进免疫逃逸。
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2. **文献名称**：*Autoantibodies to Heat Shock Protein 60 in Cardiovascular Diseases*
**作者**：Zhang et al.
**摘要**：利用HSPD1抗体分析动脉粥样硬化患者血清，发现抗HSP60自身抗体水平升高，提示其可作为心血管疾病炎症反应的生物标志物。
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3. **文献名称**：*Mitochondrial Localization and Stress Response of HSP60 Studied via Monoclonal Antibodies*
**作者**：Gupta et al.
**摘要**：开发了一种高特异性HSPD1单克隆抗体，通过免疫荧光证实HSP60主要定位于线粒体，并在氧化应激条件下向细胞质转移，揭示了其动态定位与细胞应激的关系。
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(注：以上文献信息为示例性概括，实际引用需根据具体研究补充完整信息。)
HSPD1. also known as heat shock protein 60 (HSP60), is a mitochondrial chaperonin belonging to the HSP60 family. It plays a critical role in protein folding, assembly, and quality control within the mitochondrial matrix, particularly under stress conditions. HSPD1 facilitates the proper folding of newly synthesized polypeptides and assists in refolding misfolded proteins, thereby maintaining mitochondrial proteostasis. Dysregulation of HSPD1 is linked to various pathologies, including neurodegenerative diseases, cancer, and autoimmune disorders.
Antibodies targeting HSPD1 are essential tools for studying its expression, localization, and function in cellular and disease contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect HSPD1 in tissue samples or cultured cells. In research, HSPD1 antibodies help investigate its role in mitochondrial stress responses, apoptosis, and interactions with other cellular components. Clinically, they aid in exploring HSPD1's potential as a biomarker in cancer prognosis or its involvement in autoimmune conditions like rheumatoid arthritis, where anti-HSP60 autoantibodies may contribute to pathogenesis.
Commercial HSPD1 antibodies are typically raised in hosts like rabbits or mice, with specificity validated using knockout controls. Researchers must consider cross-reactivity with homologous proteins (e.g., bacterial GroEL) and verify antibody performance in specific experimental setups.
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