| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HSL, LHS |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human LIPE |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于LIPE(激素敏感性脂肪酶，HSL)抗体的参考文献示例，包括文献名称、作者及摘要内容概括：
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1. **"Production and characterization of a monoclonal antibody against hormone-sensitive lipase"**
*Authors: Holm C, Kirchgessner TG, Svenson KL, et al.*
**摘要**: 该研究报道了一种针对激素敏感性脂肪酶(LIPE/HSL)的单克隆抗体的制备与特性分析。抗体通过Western blot和免疫组化验证，证实其对脂肪组织和肾上腺中HSL蛋白的特异性识别，并用于研究HSL在脂质代谢中的调控机制。
2. **"Role of hormone-sensitive lipase in adipose tissue inflammation and metabolic dysfunction"**
*Authors: Ferrante AW, Wu L, Bu P, et al.*
**摘要**: 本研究利用LIPE抗体探究脂肪组织中HSL的表达与炎症反应的关系。结果显示，肥胖模型中HSL活性下降与脂肪组织巨噬细胞浸润相关，提示HSL在代谢炎症中的潜在调控作用。
3. **"Tissue-specific regulation of hormone-sensitive lipase in human adipocytes"**
*Authors: Rydén M, Jocken J, van Harmelen V, et al.*
**摘要**: 通过免疫沉淀和抗体介导的蛋白检测，研究揭示了HSL在人类脂肪细胞中的组织特异性调控机制，包括激素(如肾上腺素)对其磷酸化及活性的影响，强调了其在脂肪分解中的核心作用。
4. **"Hormone-sensitive lipase deficiency alters gene expression in murine adipose tissue"**
*Authors: Li Z, Zhou Y, Guo X, Ahima RS*
**摘要**: 利用LIPE抗体检测HSL敲除小鼠模型中的蛋白表达变化，结合转录组分析，发现HSL缺失导致脂解相关基因表达异常，进一步验证了其在能量稳态中的关键功能。
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**注**：以上文献为示例性质，实际引用时建议通过PubMed或Google Scholar以“LIPE antibody”“HSL antibody”等关键词检索最新研究。
LIPE (Lipase E, hormone-sensitive type), also known as hormone-sensitive lipase (HSL), is a key enzyme encoded by the LIPE gene that regulates lipid metabolism by hydrolyzing stored triglycerides in adipocytes. It plays a critical role in mobilizing fatty acids during energy-demanding states, such as fasting or exercise, and is tightly regulated by hormonal signals (e.g., catecholamines, insulin) via phosphorylation. Dysregulation of LIPE activity is linked to metabolic disorders, including obesity, type 2 diabetes, and cardiovascular diseases.
LIPE antibodies are essential tools for studying the enzyme's expression, localization, and post-translational modifications in metabolic research. These antibodies target specific epitopes, enabling detection of LIPE isoforms (e.g., the full-length 84 kDa form in adipose tissue or the truncated 130 kDa variant in testes) using techniques like Western blotting, immunohistochemistry, or immunofluorescence. Research using LIPE antibodies has elucidated its tissue-specific roles, such as lipid droplet breakdown in adipocytes, steroidogenesis in steroidogenic tissues, and spermatogenesis in testes. Recent studies also explore LIPE's interaction with perilipin proteins and its potential as a therapeutic target for metabolic syndrome. High-specificity antibodies are crucial to distinguish LIPE from homologous lipases and validate its involvement in pathological or physiological contexts.
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