| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | dJ1007G16.5 |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human HMGB4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HMGB4抗体的3篇示例参考文献(注：以下内容为模拟示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: "Developmental regulation and functional characterization of HMGB4 in mammalian spermatogenesis"
**作者**: Chen L, et al.
**摘要**: 本研究通过制备特异性HMGB4抗体，揭示了其在哺乳动物睾丸组织中的动态表达模式，并发现HMGB4通过调控染色质重塑参与精子细胞分化过程。抗体验证显示其在生殖细胞核中特异性富集。
2. **文献名称**: "HMGB4 monoclonal antibody generation and its application in ovarian germ cell tumors"
**作者**: Tanaka K, et al.
**摘要**: 文章报道了一种新型HMGB4单克隆抗体的开发，通过免疫印迹和免疫组化验证其高特异性。该抗体被用于检测卵巢生殖细胞肿瘤中HMGB4的异常表达，提示其作为潜在生物标志物的价值。
3. **文献名称**: "The role of HMGB4 in chromatin stability during early embryonic development"
**作者**: Müller S, et al.
**摘要**: 利用HMGB4抗体进行免疫荧光和ChIP-seq分析，研究发现HMGB4在早期胚胎中维持染色质结构完整性，敲除实验导致胚胎发育停滞。抗体特异性通过基因沉默实验进一步确认。
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建议通过PubMed或Web of Science以“HMGB4 antibody”为关键词检索最新文献，或关注《Journal of Biological Chemistry》《Development》等期刊相关研究。实际研究中需严格验证抗体特异性(如敲除/敲低实验对照)。
High-mobility group box 4 (HMGB4) is a member of the HMGB protein family, which plays roles in DNA organization, transcriptional regulation, and chromatin dynamics. Unlike other HMGB proteins (e.g., HMGB1-3), HMGB4 exhibits tissue-specific expression, predominantly in the testes, and is implicated in spermatogenesis and male germ cell development. It binds DNA with lower affinity than other HMGB proteins, suggesting unique regulatory functions, potentially in chromatin remodeling during gametogenesis or post-meiotic stages of sperm maturation.
HMGB4 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate HMGB4's role in reproductive biology and germ cell-specific processes. Research has linked HMGB4 to fertility, as its deficiency in mice leads to impaired spermiogenesis and reduced fertility. However, its precise mechanisms remain less understood compared to other HMGB members, highlighting the need for reliable antibodies to explore its functions.
Commercial HMGB4 antibodies are typically developed using immunogenic peptides or recombinant proteins, with validation in specific applications and species (e.g., human, mouse). Researchers must verify antibody specificity due to potential cross-reactivity with other HMGB family proteins. Ongoing studies aim to clarify HMGB4's contributions to cellular differentiation, epigenetic regulation, and potential associations with infertility or testicular pathologies, underscoring its emerging significance in reproductive medicine.
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