| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HIRS-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human IRS1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于IRS1抗体的参考文献示例(注：以下内容为示例性构造，建议通过学术数据库检索最新文献)：
1. **文献名称**：*"IRS-1 Antibodies as Biomarkers in Type 2 Diabetes and Insulin Resistance"*
**作者**：Smith A, et al.
**摘要**：研究利用IRS1抗体检测糖尿病患者外周血中IRS1的磷酸化状态，发现IRS1 Ser307位点的异常磷酸化与胰岛素抵抗显著相关，提示其作为糖尿病诊断的潜在标志物。
2. **文献名称**：*"Role of IRS1 Antibodies in Cancer Cell Signaling Pathways"*
**作者**：Brown L, et al.
**摘要**：通过IRS1抗体阻断实验，验证了IRS1在乳腺癌细胞中通过PI3K/AKT通路调控肿瘤增殖的机制，表明靶向IRS1可能成为癌症治疗的新策略。
3. **文献名称**：*"Autoantibodies Against IRS1 in Autoimmune Disorders"*
**作者**：Zhang Y, et al.
**摘要**：首次报道系统性红斑狼疮(SLE)患者血清中存在IRS1自身抗体，可能通过干扰胰岛素信号通路参与代谢异常和自身免疫病理过程。
4. **文献名称**：*"IRS1 Phosphorylation Dynamics Studied by Monoclonal Antibodies"*
**作者**：Johnson R, et al.
**摘要**：开发了特异性识别IRS1不同磷酸化位点的单克隆抗体，并应用于肥胖模型小鼠组织分析，揭示了IRS1在代谢调控中的时空动态变化。
**建议**：可通过PubMed、Google Scholar等平台以关键词“IRS1 antibody”“IRS1 phosphorylation”“IRS1 biomarker”检索近五年文献获取最新进展。
IRS1 (Insulin Receptor Substrate 1) antibodies are tools used to study the role of IRS1. a key adaptor protein in insulin and insulin-like growth factor (IGF) signaling pathways. IRS1 mediates signal transduction by binding phosphorylated tyrosine residues on activated insulin/IGF receptors, initiating downstream cascades that regulate glucose metabolism, cell growth, and survival. Dysregulation of IRS1 is linked to insulin resistance, type 2 diabetes, and cancer.
Antibodies targeting IRS1 are widely employed in research to detect its expression, phosphorylation status (e.g., at Ser307 or Tyr612), and subcellular localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Phospho-specific antibodies help assess IRS1 activation or inhibition under varying metabolic or pathological conditions.
In clinical contexts, autoantibodies against IRS1 have been detected in certain autoimmune or metabolic disorders. For instance, IRS1 autoantibodies may impair insulin signaling, contributing to insulin resistance in type 2 diabetes or polycystic ovary syndrome (PCOS). Research also explores their potential as biomarkers for disease progression or therapeutic targeting.
Overall, IRS1 antibodies serve as critical reagents for unraveling molecular mechanisms in metabolic diseases and cancer, while their detection in autoimmunity highlights their dual role in both research and clinical diagnostics.
×
