| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2 |
| WB Predicted band size | 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human SNAP25 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于SNAP25抗体的文献概览(虚构标题，供参考)：
1. **《SNAP25 isoforms differentially regulate synaptic vesicle exocytosis》**
- 作者：Bark, C. et al.
- 摘要：通过特异性抗体区分SNAP25的两种剪接变体(SNAP25a/b)，研究其在神经元和内分泌细胞分泌中的功能差异，发现不同亚型对钙离子敏感性和融合动力学的影响。
2. **《Autoantibodies to SNAP25 in paraneoplastic neurological syndromes》**
- 作者：Dalmau, J. et al.
- 摘要：报道SNAP25抗体作为副肿瘤性小脑变性等神经综合征的生物标志物，通过患者血清抗体与脑组织SNAP25蛋白的免疫反应性关联临床症状。
3. **《SNAP25 deletion in mice alters presynaptic plasticity》**
- 作者：Washbourne, P. et al.
- 摘要：利用SNAP25抗体验证基因敲除小鼠模型中的蛋白缺失，证明SNAP25缺失导致突触小泡循环异常和短期可塑性受损，支持其在神经传递中的核心作用。
4. **《Mapping SNARE complex assembly with domain-specific antibodies》**
- 作者：Jahn, R. & Südhof, T.C.
- 摘要：通过针对SNAP25不同结构域的单克隆抗体，解析其与syntaxin、synaptobrevin形成SNARE复合体的动态过程，揭示突触融合的分子机制。
(注：以上文献标题和作者为示例性质，实际文献需通过PubMed等平台检索验证。)
The SNAP25 (Synaptosomal-Associated Protein 25) antibody is a key tool for studying synaptic function and neurodevelopmental disorders. SNAP25. a member of the SNARE protein family, facilitates vesicle docking and fusion by forming a complex with syntaxin and synaptobrevin, enabling neurotransmitter release. It exists in two splice variants, SNAP25a and SNAP25b, with differential expression during brain development. Dysregulation of SNAP25 is linked to neurological and psychiatric conditions, including Alzheimer’s disease, schizophrenia, and attention-deficit/hyperactivity disorder (ADHD).
SNAP25 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and interactions in neuronal tissues or cultured cells. They help investigate synaptic plasticity, exocytosis mechanisms, and disease-related alterations. Specific antibodies targeting distinct isoforms or post-translationally modified forms (e.g., phosphorylated SNAP25) further elucidate functional diversity.
Research using SNAP25 antibodies has also explored non-neuronal roles, such as hormone secretion in endocrine cells. Their applications extend to diagnostics and drug development, particularly for disorders with synaptic dysfunction. Validation of antibody specificity remains critical, as cross-reactivity with homologous SNARE proteins can occur. Overall, SNAP25 antibodies are indispensable for unraveling synaptic biology and pathophysiological mechanisms in neurological diseases.
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