首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述:兔子纤溶酶原(Plg)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的PLG含量。PLG即纤溶酶原,是纤溶系统的关键成分。其可被激活转变为纤溶酶,参与溶解纤维蛋白凝块。研究聚焦于其激活机制以及与血栓形成、溶解的关联,探索以PLG为靶点开发防治血栓性疾病、癌症转移等病症的药物。试剂盒检测范围为62.5 ng/mL-4000 ng/mL,灵敏度为15.6 ng/mL。科研人员可将其应用于动物模型的血栓形成研究、药物干预后纤溶功能评估或相关疾病分子机制探索,尤其适合需要高灵敏度检测小体积样本(如微量血浆)的实验设计,为研究纤溶系统调控及病理生理关联提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Plasmin ELISA Kit; Plasmin heavy chain A ELISA Kit; Plasmin light chain B ELISA Kit; Plasminogen ELISA Kit; PLG ELISA Kit; PLMN_HUMAN ELISA Kit
缩写:PLG
Uniprot No.:P00747
种属:Oryctolagus cuniculus (Rabbit)
样本类型:serum, plasma, tissue homogenates
检测范围:62.5 ng/mL-4000 ng/mL
灵敏度:15.6 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cardiovascular
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Plg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 92 | |||||
Range % | 86-103 | ||||||
1:2 | Average % | 89 | |||||
Range % | 85-99 | ||||||
1:4 | Average % | 95 | |||||
Range % | 88-97 | ||||||
1:8 | Average % | 90 | |||||
Range % | 83-100 |
回收率:
The recovery of rabbit Plg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 96 | 88-102 | |||||
EDTA plasma (n=4) | 97 | 83-100 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
40000.1130.1150.114 20000.2160.2270.222 10000.4670.4720.470 5000.7850.7920.789 2501.2181.2201.219 1251.6821.6931.688 62.52.1592.1722.166 02.6002.6292.615 |
货期:3-5 working days
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