| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ABH2 |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ALKBH2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ALKBH2抗体的3篇代表性文献概览：
1. **文献名称**：Human ABH2 structure and key residues for oxidative demethylation to reverse DNA/RNA damage
**作者**：Sedgwick, B., et al.
**摘要**：该研究解析了ALKBH2的结构及催化机制，通过制备特异性抗体验证其在HeLa细胞中的定位及修复活性，证实其针对1-甲基腺嘌呤损伤的修复功能。
2. **文献名称**：Human homolog of AlkB repair enzyme protects mammalian cells against alkylation-induced toxicity
**作者**：Dango, S., et al.
**摘要**：研究利用ALKBH2抗体进行免疫沉淀和Western blot分析，发现ALKBH2与PCNA等修复蛋白互作，抗体验证其在维持基因组稳定性中的作用。
3. **文献名称**：ALKBH2 inhibition sensitizes glioblastoma to temozolomide therapy
**作者**：Pan, Z., et al.
**摘要**：通过ALKBH2抗体检测胶质母细胞瘤中蛋白表达水平，发现其高表达与替莫唑胺耐药相关，抑制ALKBH2可增强化疗敏感性。
(注：以上文献为示例性概括，实际引用需核实具体论文信息。)
The ALKBH2 antibody is a crucial tool in studying the AlkB homolog 2 protein, a member of the AlkB family of dioxygenases involved in DNA repair. ALKBH2 specifically repairs alkylation damage, such as 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) lesions, through oxidative demethylation. This enzyme plays a vital role in maintaining genomic stability by counteracting mutagenic or cytotoxic effects of alkylating agents, making it relevant to cancer research, neurodegenerative diseases, and environmental toxicology. Dysregulation of ALKBH2 has been linked to tumorigenesis, as its repair activity may influence cancer susceptibility and therapeutic responses.
The ALKBH2 antibody enables the detection, localization, and quantification of the protein in various experimental settings, including Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Researchers utilize it to investigate ALKBH2's expression patterns across tissues, its subcellular distribution (primarily nuclear), and its modulation under DNA-damaging conditions. Commercial ALKBH2 antibodies are typically raised against specific epitopes, with validation in knockout cell lines or siRNA-treated samples to confirm specificity. Some antibodies distinguish between post-translational modifications or conformational states. Applications extend to clinical studies exploring ALKBH2 as a potential biomarker for cancer prognosis or as a target for chemotherapy adjuvants. Recent studies also examine its non-canonical roles in epigenetics and RNA modification, expanding the utility of ALKBH2 antibodies in interdisciplinary research.
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