| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | ABH; ABH1; alkB; hABH; ALKBH |
| WB Predicted band size | 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ALKBH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于ALKBH1抗体的参考文献摘要：
1. **"ALKBH1-mediated tRNA demethylation regulates translation"**
*作者：Liu F. et al. (2016)*
摘要：研究报道ALKBH1通过去甲基化tRNA上的m1A修饰调控翻译过程。使用ALKBH1抗体进行免疫沉淀和蛋白质印迹实验，发现其在小鼠胚胎干细胞中调控核糖体生物合成和胚胎发育。
2. **"Mitochondrial ALKBH1 repairs methylated RNA and DNA damage"**
*作者：Kawarada L. et al. (2017)*
摘要：研究发现ALKBH1定位于线粒体，利用特异性抗体(如Western blot和免疫荧光)证实其通过修复甲基化损伤的RNA和线粒体DNA，维持线粒体功能，并影响细胞氧化应激反应。
3. **"ALKBH1 is required for maintenance of pluripotency in human embryonic stem cells"**
*作者：Wu T.P. et al. (2018)*
摘要：通过ALKBH1抗体进行ChIP-seq分析，揭示ALKBH1通过调控DNA羟甲基化修饰(5hmC)维持人多能干细胞干性，敲低ALKBH1导致分化相关基因异常激活。
4. **"Structural basis of ALKBH1 recognizing substrates for oxidative demethylation"**
*作者：Yi C. et al. (2019)*
摘要：利用ALKBH1抗体纯化蛋白并进行结构解析，阐明其识别单链DNA/RNA中甲基化损伤的分子机制，揭示其底物选择性和酶活调控的关键位点。
这些研究均通过ALKBH1抗体验证其表达、定位及功能，涵盖发育、翻译调控和损伤修复等领域。
ALKBH1 (AlkB homolog 1) is a member of the AlkB family of α-ketoglutarate-dependent dioxygenases, which are involved in nucleic acid repair and epigenetic regulation. It primarily functions as a demethylase targeting various DNA and RNA modifications, including N6-methyladenine (m6A) and N1-methyladenine (m1A) in DNA, as well as specific RNA modifications. ALKBH1 plays critical roles in transcriptional regulation, mitochondrial function, and genome stability, with implications in development, stem cell biology, and disease. Its enzymatic activity is closely associated with oxidative demethylation processes that influence chromatin structure and gene expression.
Antibodies against ALKBH1 are essential tools for studying its expression, localization, and molecular interactions. These antibodies are widely used in techniques such as Western blotting, immunoprecipitation (IP), and immunofluorescence (IF) to investigate ALKBH1's role in DNA/RNA repair, epigenetic modifications, and cellular stress responses. Specific monoclonal or polyclonal ALKBH1 antibodies are validated for species reactivity (e.g., human, mouse, rat) and application-specific compatibility. Researchers often employ knock-out cell lines or tissues to confirm antibody specificity. Dysregulation of ALKBH1 has been linked to cancer progression, neurological disorders, and mitochondrial diseases, making its antibodies valuable for exploring disease mechanisms and therapeutic targets. Recent studies also highlight its potential involvement in regulating tRNA modifications and translational efficiency, expanding its relevance in post-transcriptional gene regulation.
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