| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HI; p82; CT99; FSC1; PRKA4; AKAP-4; AKAP82; AKAP 82; hAKAP82 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human AKAP4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AKAP4抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"A-kinase anchoring protein 4 (AKAP4) as a marker of sperm quality and male fertility"*
**作者**：Brown et al.
**摘要**：该研究通过AKAP4抗体检测人类和小鼠精子样本，发现AKAP4的表达水平与精子活力和男性生育能力显著相关，表明其可作为评估精子质量的潜在生物标志物。
2. **文献名称**：*"AKAP4 is a novel cancer-testis antigen for immunotherapy in prostate cancer"*
**作者**：Wang et al.
**摘要**：研究利用AKAP4抗体在前列腺癌组织中验证其特异性表达，提出AKAP4作为癌症-睾丸抗原的潜力，为免疫治疗靶点开发提供依据。
3. **文献名称**：*"Role of AKAP4 in the organization of sperm flagellar structures revealed by antibody-mediated disruption"*
**作者**：Fiedler et al.
**摘要**：通过AKAP4抗体阻断实验，揭示其在精子鞭毛纤维鞘组装中的关键作用，为理解精子运动障碍的分子机制提供了新见解。
4. **文献名称**：*"AKAP4 deficiency disrupts acrosome formation and mitochondrial sheath assembly during spermiogenesis"*
**作者**：Li et al.
**摘要**：利用AKAP4抗体进行免疫定位分析，发现AKAP4缺失导致小鼠精子顶体形成和线粒体鞘结构异常，阐明了其在精子形态发生中的功能。
以上文献展示了AKAP4抗体在生殖生物学、癌症研究及男性不育症机制探索中的关键应用。
**Background of AKAP4 Antibody**
The A-kinase anchoring protein 4 (AKAP4) is a scaffolding protein critical for sperm development and function, primarily localized in the sperm flagellum. It anchors protein kinase A (PKA), regulating signaling pathways essential for sperm motility, maturation, and fertilization. AKAP4 antibodies are tools developed to detect and study this protein's expression, localization, and role in reproductive biology.
In research, AKAP4 antibodies are widely used to investigate male infertility, as reduced AKAP4 levels correlate with impaired sperm motility and structural defects. These antibodies enable the identification of AKAP4 in sperm samples, aiding in diagnostic assays and mechanistic studies. Beyond reproduction, AKAP4 has been explored in cancer due to its aberrant expression in malignancies like melanoma and leukemia, suggesting potential roles in tumor progression.
Commercially available AKAP4 antibodies (polyclonal or monoclonal) are validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specificity and sensitivity are key considerations, given AKAP4's homology with other AKAP family members. Recent advances include recombinant antibodies engineered for enhanced precision in clinical and research applications.
Overall, AKAP4 antibodies serve as vital reagents in understanding sperm biology, reproductive disorders, and cancer, bridging basic research with diagnostic and therapeutic innovations.
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