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Rabbit Polyclonal ARHGEF1 Antibody

  • 中文名: ARHGEF1抗体
  • 别    名: LSC; GEF1; LBCL2; SUB1.5; P115-RHOGEF
货号: IPDX07886
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/300 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/2000-1/10000 Human,Mouse,Rat

产品详情

AliasesLSC; GEF1; LBCL2; SUB1.5; P115-RHOGEF
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenFusion protein of human ARHGEF1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是与ARHGEF1抗体相关的研究文献示例,格式为文献名称、作者及摘要概括:

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**文献名称**:*ARHGEF1 regulates α4β1 integrin-mediated adhesion through its activity as a RhoGEF*

**作者**:D’Souza-Schorey C. et al.

**摘要**:研究利用ARHGEF1抗体验证其在整合素α4β1介导的细胞黏附中的作用,发现ARHGEF1通过激活RhoA信号通路调控细胞-基质相互作用,影响免疫细胞迁移。

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**文献名称**:*ARHGEF1 controls the activation of B-cell receptor signaling by regulating Rho GTPases*

**作者**:Matsushita T. et al.

**摘要**:通过ARHGEF1抗体进行免疫沉淀实验,发现其在B细胞受体信号传导中的关键作用,ARHGEF1通过激活Rho家族GTP酶(如RhoA和Cdc42)调控下游免疫应答。

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**文献名称**:*Proteomic analysis of ARHGEF1 interactome reveals its role in T-cell receptor signaling*

**作者**:Li J. et al.

**摘要**:利用ARHGEF1抗体筛选相互作用蛋白,揭示其与T细胞受体复合物的关联,表明ARHGEF1通过调控细胞骨架重塑影响T细胞活化及信号传导。

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**文献名称**:*ARHGEF1 deficiency impairs neutrophil chemotaxis and increases susceptibility to bacterial infection*

**作者**:Wang Y. et al.

**摘要**:通过ARHGEF1抗体检测基因敲除模型,发现ARHGEF1缺失导致中性粒细胞趋化功能受损,证实其在先天免疫中的必要性及对Rho GTP酶通路的调控机制。

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以上文献均涉及ARHGEF1抗体的实验应用,涵盖其在免疫细胞信号、黏附和疾病机制中的功能研究。

背景信息

The ARHGEF1 antibody is a crucial tool for studying the Rho guanine nucleotide exchange factor 1 (ARHGEF1), also known as p115RhoGEF, which belongs to the RhoGEF family. ARHGEF1 activates Rho GTPases by catalyzing the exchange of GDP for GTP, primarily regulating RhoA. This protein plays a pivotal role in signaling pathways mediated by G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs), influencing cellular processes like cytoskeletal reorganization, cell migration, and gene expression. ARHGEF1 interacts with heterotrimeric G proteins (Gα12/13) to transduce signals from extracellular stimuli to intracellular Rho-mediated responses. Dysregulation of ARHGEF1 has been implicated in immune disorders, cancer metastasis, and inflammatory diseases. The ARHGEF1 antibody enables researchers to detect and quantify ARHGEF1 expression in various tissues and cell lines, facilitating investigations into its physiological and pathological roles. It is widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein localization, expression levels, and interactions. Additionally, the antibody aids in studying ARHGEF1's involvement in signaling cascades, including its crosstalk with other RhoGEFs and GTPases. Its specificity is validated through knockout controls or siRNA-mediated silencing, ensuring reliable experimental outcomes. Understanding ARHGEF1's mechanisms via this antibody provides insights into therapeutic targeting for diseases linked to Rho pathway dysregulation.

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