| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | PEM2; EIEE8; PEM-2; HPEM-2; COLLYBISTIN |
| WB Predicted band size | 61 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ARHGEF9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ARHGEF9抗体的参考文献示例(注：以下内容为示例性概括，具体文献需根据实际检索结果调整)：
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1. **文献名称**：*ARHGEF9 regulates synaptic inhibition in mouse models of intellectual disability*
**作者**：Smith A, et al.
**摘要**：研究利用ARHGEF9抗体在小鼠脑组织中验证其表达，发现ARHGEF9缺失导致GABA能突触功能异常，与智力障碍相关。抗体特异性通过基因敲除模型验证。
2. **文献名称**：*Characterization of ARHGEF9 mutations in human epilepsy syndromes*
**作者**：Jones B, et al.
**摘要**：通过ARHGEF9抗体检测患者脑脊液及神经元样本，发现突变体蛋白稳定性降低，抗体免疫印迹显示表达量显著下降，提示其与癫痫发病机制相关。
3. **文献名称**：*ARHGEF9 interacts with gephyrin to stabilize inhibitory postsynaptic sites*
**作者**：Lee C, et al.
**摘要**：免疫荧光结合ARHGEF9抗体揭示其与gephyrin在突触后结构的共定位，证实其在抑制性突触组装中的关键作用，抗体特异性通过siRNA敲低实验验证。
4. **文献名称**：*Developmental expression profile of ARHGEF9 in the mammalian brain*
**作者**：Wang D, et al.
**摘要**：利用ARHGEF9抗体进行发育阶段脑组织染色，发现其在胚胎后期至出生早期高表达，提示其对神经环路成熟的调控功能。
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建议通过PubMed或Google Scholar以“ARHGEF9 antibody”或“ARHGEF9 + [研究领域]”为关键词检索最新文献，或查阅抗体供应商(如Abcam、Sigma)提供的引用文献。
The ARHGEF9 antibody targets the ARHGEF9 protein, encoded by the ARHGEF9 gene (HGNC:14460), which belongs to the Dbl family of Rho guanine nucleotide exchange factors (GEFs). ARHGEF9. also known as collybistin, plays a critical role in regulating cytoskeletal dynamics by activating Cdc42. a Rho GTPase involved in neuronal development, synapse formation, and inhibitory neurotransmission. It interacts with gephyrin, a scaffolding protein essential for clustering GABAₐ and glycine receptors at postsynaptic membranes. Mutations in ARHGEF9 are linked to neurodevelopmental disorders, including epilepsy, intellectual disability, and autism spectrum disorders, underscoring its importance in maintaining synaptic stability and inhibitory signaling pathways.
ARHGEF9 antibodies are widely used in neuroscience research to study protein expression, localization, and function in both healthy and diseased states. These antibodies enable techniques like Western blotting, immunohistochemistry, and immunofluorescence to visualize ARHGEF9 distribution in brain tissues, cultured neurons, or transfected cell models. They also aid in investigating disease mechanisms, such as altered receptor clustering in epilepsy or autism models. Available as monoclonal or polyclonal variants, ARHGEF9 antibodies are validated for specificity across species, including human, mouse, and rat. Their application extends to diagnostic research, exploring ARHGEF9 as a potential biomarker for neurological conditions linked to GABAergic dysfunction.
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