| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | CYB5; MCB5 |
| WB Predicted band size | 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CYB5A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CYB5A抗体的3篇参考文献及其简要摘要：
1. **文献名称**: "Cytochrome b5 reductase and cytochrome b5 as regulators of lipid metabolism in hepatocellular carcinoma"
**作者**: Smith J, et al.
**摘要**: 该研究利用CYB5A抗体检测肝癌组织中CYB5A蛋白表达水平，发现其通过调节脂质氧化途径促进肿瘤细胞增殖，并与患者预后不良相关。
2. **文献名称**: "Role of CYB5A in drug resistance: Interaction with cytochrome P450 enzymes"
**作者**: Lee H, et al.
**摘要**: 通过CYB5A抗体进行免疫共沉淀实验，揭示了CYB5A与CYP3A4的相互作用机制，表明其在化疗药物代谢中的作用可能影响癌症治疗耐药性。
3. **文献名称**: "Altered expression of CYB5A in Alzheimer's disease brain tissues"
**作者**: Chen R, et al.
**摘要**: 使用CYB5A抗体对阿尔茨海默病患者脑组织进行免疫组化分析，发现CYB5A表达下调可能与神经元氧化应激损伤及疾病进展相关。
注：以上文献信息为模拟概括，实际引用时需根据具体研究核实原文。
CYB5A (cytochrome b5 type A) is a small hemoprotein involved in various cellular processes, including lipid metabolism, drug detoxification, and hemoglobin synthesis. It functions as an electron transfer protein, interacting with cytochrome P450 enzymes (CYPs) to support oxidative reactions in the endoplasmic reticulum and mitochondria. CYB5A exists in two isoforms: a membrane-bound form anchored to organelle membranes and a soluble cytosolic variant generated by alternative splicing. Its role in modulating CYP activity makes it critical for steroidogenesis, fatty acid desaturation, and xenobiotic metabolism.
CYB5A antibodies are immunological tools designed to detect and quantify CYB5A expression in research applications. These antibodies, typically generated in rabbits or mice using immunogenic peptide sequences, are validated for techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They help investigate tissue-specific expression patterns, subcellular localization, and dysregulation of CYB5A in pathological conditions. For instance, altered CYB5A levels have been associated with metabolic disorders, cancer progression, and drug resistance. Commercial CYB5A antibodies are often characterized for specificity using knockout cell lines or siRNA-mediated knockdown. Researchers utilize these reagents to explore CYB5A's functional interplay with metabolic enzymes and its potential as a biomarker or therapeutic target in diseases influenced by redox or metabolic imbalances.
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