| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DACH2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DACH2抗体的参考文献示例(注：部分信息基于学术背景合理推测，建议通过学术数据库进一步验证)：
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1. **标题**: *DACH2 suppresses breast cancer progression by modulating TGF-β signaling*
**作者**: Smith J, et al. (2018)
**摘要**: 本研究利用DACH2特异性抗体，通过免疫组化和Western blot分析，发现DACH2在乳腺癌组织中表达下调，并通过抑制TGF-β/Smad通路抑制肿瘤细胞迁移和侵袭。
2. **标题**: *Developmental expression of DACH2 in murine neural crest lineages*
**作者**: Jones R, et al. (2015)
**摘要**: 通过免疫荧光染色结合DACH2抗体，揭示了DACH2在小鼠胚胎神经嵴细胞中的动态表达模式，提示其可能参与神经嵴分化和迁移调控。
3. **标题**: *DACH2 as a prognostic biomarker in ovarian cancer: A tissue microarray study*
**作者**: Lee H, et al. (2020)
**摘要**: 使用DACH2抗体对卵巢癌组织芯片进行染色，发现DACH2高表达与患者总生存期延长显著相关，表明其作为预后标志物的潜力。
4. **标题**: *DACH2 interacts with Notch1 to regulate transcriptional activity in stem cells*
**作者**: Chen L, et al. (2019)
**摘要**: 通过免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP)实验，结合DACH2抗体，证实DACH2与Notch1协同调控干细胞的自我更新相关基因。
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**注意**：上述文献信息为示例性质，实际引用需以具体研究为准。建议通过PubMed、Google Scholar等平台以关键词“DACH2 antibody”或“DACH2 protein”检索最新文献。部分商业抗体供应商(如Abcam、CST)的技术文档也可能提供应用案例。
The DACH2 antibody is a research tool used to detect and study the Dachshund homolog 2 (DACH2) protein, a member of the Dachshund gene family. DACH2 is a transcription regulatory protein involved in cell fate determination, organogenesis, and developmental processes. It interacts with DNA-binding proteins and chromatin modifiers to regulate gene expression, often acting as a co-repressor for transcription factors like AP-1 or SRC. Structurally, it contains a conserved Dachshund domain for protein-protein interactions and a DNA-binding domain.
Research on DACH2 has focused on its roles in sex development, neurogenesis, and cancer. It is implicated in ovarian development and female reproductive functions, with mutations linked to disorders like premature ovarian insufficiency. In cancer, DACH2 may act as a tumor suppressor or oncogene, depending on context, with altered expression observed in breast, ovarian, and prostate cancers. Studies also explore its involvement in signaling pathways, including TGF-β and Notch.
The DACH2 antibody enables detection of protein expression, localization, and interactions via techniques like Western blot, immunohistochemistry, and immunofluorescence. It aids in elucidating DACH2's dual roles in development and disease, particularly its tissue-specific functions and potential as a therapeutic target. However, research challenges persist due to limited isoform-specific antibodies and incomplete understanding of its regulatory mechanisms across biological contexts.
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