| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEURL2 |
| Uniprot No | Q9BR09 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-285aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAAASEPVDSGALWGLERPEPPPTRFH RVHGANIRVDPSGTRATRVESFAHGVCFSREPLAPGQVFLVEIEEKELGW CGHLRLGLTALDPASLAPVPEFSLPDLVNLGHTWVFAITRHHNRVPREGR PEAEAAAPSRPPTLLVEPYLRIEQFRIPRDRLVGRSRPGLYSHLLDQLYE LNVLPPTARRSRLGVLFCPRPDGTADMHIIINGEDMGPSARGLPAAQPLY AVVDVFASTKSVRLVQLEYGLPSLQTLCRLVIQRSMVHRLAIDGLHLPKE LKDFCKYE |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NEURL2重组蛋白的3篇参考文献及其摘要内容的简要总结：
1. **文献名称**：*NEURL2 mediates Notch signaling to promote tumorigenesis through ubiquitination*
**作者**：Li Y, et al.
**摘要**：该研究揭示了NEURL2重组蛋白通过泛素化修饰调控Notch信号通路的机制，发现其在多种癌症中异常高表达，促进肿瘤细胞增殖和侵袭。作者通过体外重组蛋白实验证实NEURL2直接结合并泛素化Notch受体，增强其稳定性。
2. **文献名称**：*Structural and functional analysis of recombinant NEURL2 in neural development*
**作者**：Wang X, et al.
**摘要**：本文解析了重组NEURL2蛋白的晶体结构，发现其C端结构域对神经元分化至关重要。实验表明，NEURL2通过调控突触蛋白的泛素化降解，影响斑马鱼模型的神经发育过程。
3. **文献名称**：*Recombinant NEURL2 as a novel biomarker for cardiac hypertrophy*
**作者**：Saito K, et al.
**摘要**：研究团队利用重组NEURL2蛋白开发了高灵敏度检测方法，发现其在心肌肥厚患者血清中显著上调。动物实验表明，NEURL2通过调节钙调蛋白通路参与病理性心脏重构。
注：以上文献信息为模拟示例，实际研究中请通过PubMed、Web of Science等平台检索真实文献。
Neuralized E3 ubiquitin-protein ligase 2 (NEURL2) is a member of the Neuralized family of E3 ubiquitin ligases, which play critical roles in regulating protein turnover through the ubiquitin-proteasome system. This enzyme catalyzes the transfer of ubiquitin molecules to specific substrate proteins, marking them for degradation or functional modification. NEURL2 is evolutionarily conserved and implicated in diverse cellular processes, including neurodevelopment, cell cycle regulation, and signaling pathways such as the Notch and Hedgehog cascades. Its involvement in these pathways underscores its potential role in tissue homeostasis, differentiation, and disease pathogenesis, particularly in cancers and neurological disorders.
Recombinant NEURL2 protein is engineered using molecular cloning techniques, where the NEURL2 gene is expressed in heterologous systems like *E. coli* or mammalian cell lines (e.g., HEK293). This approach ensures high-yield production and purity, enabling functional studies. The recombinant protein retains enzymatic activity, allowing researchers to investigate its substrate specificity, interaction partners, and regulatory mechanisms in vitro. Tagging with markers such as GFP or His facilitates purification and detection.
Recent studies highlight NEURL2’s dual role in tumorigenesis, acting as either an oncogene or tumor suppressor depending on cellular context. It also influences ciliogenesis and synaptic plasticity, linking it to neurodevelopmental disorders. Recombinant NEURL2 serves as a vital tool for drug screening, structural analysis, and deciphering its role in disease mechanisms. Ongoing research aims to explore its therapeutic potential, particularly in targeting ubiquitination pathways for cancer treatment or neuroprotection.
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