| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | TRPP8; LTRPC6 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human TRPM8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与TRPM8抗体相关的文献摘要信息(基于公开数据整理，非真实文献，仅供示例参考)：
1. **"Characterization of a TRPM8-specific antibody for functional studies in sensory neurons"**
- 作者：Smith A, et al.
- 摘要：该研究验证了一种高特异性TRPM8抗体的应用，通过免疫组化和Western blot证实其在背根神经节(DRG)神经元中的表达，并证明其可用于检测冷敏感神经元中TRPM8的蛋白定位及表达水平变化。
2. **"TRPM8 expression in prostate cancer: Immunohistochemical analysis using a novel monoclonal antibody"**
- 作者：Jones R, et al.
- 摘要：报道了一种新型TRPM8单克隆抗体的开发，用于前列腺癌组织切片分析，发现TRPM8在恶性肿瘤中表达显著上调，提示其作为潜在生物标志物的价值。
3. **"Validation of anti-TRPM8 antibodies for detecting channel isoforms in cold thermoreception"**
- 作者：Lee C, et al.
- 摘要：通过比较多种TRPM8抗体的特异性，确认某抗体能选择性识别啮齿类与人类TRPM8的不同剪接变体，并应用于揭示其在皮肤冷感受器中的分布差异。
如需具体文献，建议在PubMed或Google Scholar中检索关键词“TRPM8 antibody validation”或“TRPM8 immunohistochemistry”获取最新研究。
TRPM8 (Transient Receptor Potential Melastatin 8) is a calcium-permeable ion channel belonging to the TRP superfamily, primarily known for its role in detecting cold temperatures and mediating cooling sensations. It is activated by moderate cooling (below 26°C), menthol, and icilin. Expressed in sensory neurons, prostate, and other tissues, TRPM8 regulates thermosensation, pain signaling, and cellular responses to cold. Dysregulation of TRPM8 has been linked to pathological conditions, including neuropathic pain, chronic cough, and cancer progression, making it a therapeutic target for pain management and oncology research.
TRPM8 antibodies are essential tools for studying the channel’s expression, localization, and function. They enable detection of TRPM8 in various biological samples via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). High-quality antibodies target specific epitopes, often within the channel’s N-terminal domain or pore region, and undergo validation using knockout controls to ensure specificity. Both monoclonal and polyclonal formats are available, with applications spanning basic research (e.g., mapping neuronal cold-sensing pathways) to clinical studies (e.g., analyzing TRPM8 overexpression in tumors).
Recent studies highlight TRPM8’s involvement in non-thermosensory roles, such as regulating autophagy and metabolism, expanding its relevance in metabolic disorders. Antibodies also aid in drug discovery, screening TRPM8 modulators for therapeutic potential. However, challenges persist, including cross-reactivity with related TRP channels, emphasizing the need for rigorously validated reagents. Overall, TRPM8 antibodies are critical for unraveling its multifaceted roles in physiology and disease.
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