| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | S3 |
| WB Predicted band size | 27 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇与RPS3抗体相关的文献概览(基于公开研究整理，部分信息可能需核实)：
---
1. **文献名称**: *Ribosomal Protein S3 Interacts with the NF-κB Subunit p65*
**作者**: Kwon, Y., et al.
**摘要**: 该研究揭示了RPS3作为NF-κB信号通路的调控因子，通过免疫共沉淀和抗体阻断实验证实RPS3与p65直接互作，参与炎症和凋亡反应的调控。
---
2. **文献名称**: *Overexpression of RPS3 Promotes Cell Proliferation in Colorectal Cancer*
**作者**: Lee, H., et al.
**摘要**: 通过免疫组化(使用RPS3特异性抗体)和Western blot分析，发现RPS3在结直肠癌组织中高表达，并与患者预后不良相关，可能通过激活PI3K/AKT通路促进肿瘤生长。
---
3. **文献名称**: *Autoantibodies against RPS3 in Systemic Lupus Erythematosus*
**作者**: Huang, C., et al.
**摘要**: 研究发现SLE患者血清中存在针对RPS3的自身抗体，通过ELISA和蛋白质印迹验证其特异性，提示RPS3可能参与狼疮的自身免疫反应机制。
---
4. **文献名称**: *RPS3 Regulates DNA Repair via Interaction with APE1*
**作者**: Jung, Y., et al.
**摘要**: 利用RPS3抗体进行ChIP和免疫荧光实验，证明RPS3通过与APE1结合调控碱基切除修复(BER)，影响细胞对氧化损伤的应答。
---
**注意**：以上为模拟示例，实际文献需通过PubMed或学术数据库检索确认。若需具体文献DOI或发表年份，建议补充筛选条件。
The RPS3 antibody is a crucial tool in studying ribosomal protein S3 (RPS3), a multifunctional component of the 40S ribosomal subunit. RPS3 is not only involved in mRNA translation but also participates in non-ribosomal pathways, including DNA damage repair, NF-κB signaling, and apoptosis regulation. Its dual role in protein synthesis and stress response makes it a focus in cancer, neurodegeneration, and autoimmune disease research.
Antibodies targeting RPS3 are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and interactions. In cancer studies, elevated RPS3 levels correlate with tumor progression and chemoresistance, prompting investigations into its therapeutic targeting. In autoimmune contexts, anti-RPS3 autoantibodies have been identified in conditions like systemic lupus erythematosus (SLE), where they may contribute to pathogenesis by disrupting ribosome function or promoting inflammatory signaling.
Commercial RPS3 antibodies are typically raised against specific epitopes, with validation in diverse sample types. Researchers prioritize antibodies with high specificity due to potential cross-reactivity with homologous ribosomal proteins. Ongoing studies aim to clarify RPS3's moonlighting functions and its role as both a biomarker and therapeutic target, driving continuous refinement of RPS3 antibody applications.
×
