| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | SEST3 |
| WB Predicted band size | 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是基于SESN3(Sestrin3)研究领域构造的参考文献示例，供参考：
1. **文献名称**: "Sestrin3 regulates hepatic lipid metabolism via AMPK/mTOR signaling"
**作者**: Lee, J.H., et al.
**摘要**: 本研究探讨了SESN3在肝脏脂质代谢中的调控作用，通过Western blot和免疫组化使用特异性SESN3抗体，证实其通过AMPK/mTOR通路抑制脂肪生成，为代谢性疾病提供潜在治疗靶点。
2. **文献名称**: "A novel monoclonal antibody for Sestrin3 detection in human tissues"
**作者**: Zhang, Y., et al.
**摘要**: 报道了一种高特异性SESN3单克隆抗体的开发与验证，该抗体适用于免疫印迹和免疫荧光，成功应用于多种癌症组织中SESN3蛋白的定量分析。
3. **文献名称**: "Sestrin3-mediated antioxidant defense in neuronal cells"
**作者**: Park, S., et al.
**摘要**: 研究揭示了SESN3在神经元氧化应激中的保护机制，通过抗体检测发现其通过激活Nrf2通路增强细胞抗氧化能力，为神经退行性疾病研究提供新方向。
4. **文献名称**: "Sestrin3 deficiency exacerbates cardiac hypertrophy via mTORC1 activation"
**作者**: Chen, L., et al.
**摘要**: 利用SESN3敲除模型及特异性抗体分析，证明SESN3缺失通过mTORC1信号通路促进心脏肥厚，提示其在心血管疾病中的潜在调控作用。
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**备注**：以上文献为示例性质，实际研究中建议通过学术数据库(如PubMed、Google Scholar)以关键词“Sestrin3 antibody”或“SESN3 Western blot”检索最新文献。部分真实相关研究可能包括Sestrin家族蛋白的功能探索，或特定抗体在细胞应激、代谢等领域的应用。
SESTRIN3 (SESN3) is a member of the sestrins family, evolutionarily conserved stress-inducible proteins involved in regulating cellular homeostasis under oxidative, genotoxic, and metabolic stress. Primarily studied in mammals, SESN3 functions as a redox sensor and modulator of the AMP-activated protein kinase (AMPK)-mTORC1 signaling axis, influencing processes like autophagy, mitochondrial biogenesis, and metabolism. Unlike SESN1 and SESN2. which are strongly induced by p53 during DNA damage, SESN3 expression is more tightly linked to metabolic stressors, including glucose deprivation and hypoxia. Structurally, SESN3 contains conserved cysteine residues critical for its antioxidant activity, enabling it to scavenge reactive oxygen species (ROS) and regenerate oxidized peroxiredoxins.
SESN3 antibodies are essential tools for detecting and quantifying SESN3 protein levels in research settings, particularly in studies exploring metabolic regulation, aging, and stress-response pathways. These antibodies are validated for techniques such as Western blotting, immunoprecipitation, and immunofluorescence, often targeting specific epitopes within its N- or C-terminal domains. Research applications include investigating SESN3's role in insulin resistance, lipid metabolism, and its interplay with mTORC1 in cancer or neurodegenerative diseases. Commercial SESN3 antibodies are typically raised in rabbits or mice, with cross-reactivity varying between human, mouse, and rat homologs. Recent studies highlight SESN3 as a potential therapeutic target for metabolic disorders, driving demand for high-specificity antibodies to dissect its mechanisms in disease models.
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