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Rabbit Polyclonal SHPRH Antibody

  • 中文名: SHPRH抗体
  • 别    名: bA545I5.2
货号: IPDX08765
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/30-1/150 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/2000-1/10000 Human,Mouse,Rat

产品详情

AliasesbA545I5.2
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenFusion protein of human SHPRH
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于SHPRH抗体的3篇参考文献及其摘要概括:

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1. **文献名称**:*SHPRH regulates PCNA polyubiquitination in DNA damage tolerance*

**作者**:Motegi, A. et al.

**摘要**:该研究揭示了SHPRH作为E3泛素连接酶,通过调控增殖细胞核抗原(PCNA)的多泛素化,促进DNA损伤修复。实验中利用SHPRH特异性抗体进行免疫沉淀和Western blot分析,证实其在DNA损伤应答中的关键作用。

2. **文献名称**:*SHPRH suppresses tumorigenesis through control of DNA damage response and repair in glioma*

**作者**:Tsuchiya, M. et al.

**摘要**:研究发现SHPRH在胶质瘤中作为肿瘤抑制因子,其表达降低与患者预后不良相关。通过免疫组化(使用SHPRH抗体)和功能实验,证明SHPRH缺失导致DNA修复缺陷和基因组不稳定性,促进肿瘤进展。

3. **文献名称**:*Distinct roles of SHPRH and HLTF in DNA damage tolerance mechanisms*

**作者**:Unk, I. et al.

**摘要**:本文比较了SHPRH与同源蛋白HLTF在DNA损伤耐受中的功能差异。通过抗体介导的蛋白质互作分析,发现SHPRH优先参与PCNA的K63泛素化,而HLTF偏向于其他修复途径,揭示了二者在修复机制中的分工。

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以上文献均涉及SHPRH抗体的应用(如Western blot、免疫沉淀或免疫组化),并围绕其在DNA修复或肿瘤抑制中的功能展开研究。如需更多文献或具体细节,可进一步检索PubMed或相关数据库。

背景信息

The SHPRH (SNF2 Histone Linker PHD RING Helicase) antibody is a tool used to study the SHPRH protein, a critical player in DNA damage repair and genomic stability. SHPRH, a member of the SWI/SNF family of helicases, functions as an E3 ubiquitin ligase that collaborates with the PCNA (Proliferating Cell Nuclear Antigen) to regulate error-free DNA repair pathways. It promotes the polyubiquitination of PCNA, facilitating the switch from translesion DNA synthesis to homologous recombination repair during replication stress. Discovered as a human homolog of yeast Rad5. SHPRH mutations or dysregulation have been linked to cancer susceptibility, neurodevelopmental disorders, and premature aging phenotypes. Antibodies targeting SHPRH enable researchers to investigate its expression, localization, and interactions in cellular contexts, particularly in studies involving DNA replication stress, tumorigenesis, and chemotherapeutic resistance. These antibodies are vital for techniques like Western blotting, immunofluorescence, and co-immunoprecipitation, helping to elucidate SHPRH’s role in maintaining genome integrity and its potential as a therapeutic target in cancers with defective DNA repair mechanisms.

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