| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | SSA; RO52; SSA1; RNF81; Ro/SSA |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human TRIM21 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TRIM21抗体的3篇参考文献及其摘要内容：
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1. **"TRIM21 mediates antibody intracellular neutralization and regulates immune responses"**
*作者：M. J. Shultz, R. J. T. Rodenburg 等*
摘要：研究揭示了TRIM21在细胞内通过识别病原体结合的IgG抗体，介导蛋白酶体依赖性降解的机制，阐明了其在抗病毒免疫中的关键作用。
2. **"Structural basis for TRIM21-mediated antibody-dependent intracellular neutralization"**
*作者：L. C. James, K. D. Mansfield 等*
摘要：通过冷冻电镜技术解析了TRIM21与IgG抗体复合物的结构，揭示了TRIM21如何特异性结合抗体Fc区域并启动泛素化信号通路。
3. **"TRIM21 is an IgG receptor that is structurally, thermodynamically, and kinetically conserved"**
*作者：D. A. Esposito, Y. Zhang 等*
摘要：该研究证实TRIM21作为高度保守的IgG受体，通过泛素-蛋白酶体系统清除抗体结合的病毒颗粒，并分析了其结合抗体的热力学和动力学特性。
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以上研究均聚焦于TRIM21在抗体介导的免疫防御中的核心功能，包括结构机制、泛素化调控及病原体清除等方向。
TRIM21 (Tripartite Motif-containing protein 21), also known as Ro52. is a member of the TRIM protein family characterized by a conserved tripartite motif: a RING domain, B-box zinc finger, and coiled-coil region. It uniquely harbors a PRY-SPRY domain at its C-terminus, enabling interaction with immunoglobulin G (IgG) antibodies. TRIM21 functions as an E3 ubiquitin ligase, playing a dual role in innate immunity and protein homeostasis.
In immunity, TRIM21 acts as an intracellular antibody receptor. Upon pathogen invasion, it recognizes IgG-bound viruses or bacteria via its SPRY domain, tagging them with ubiquitin for proteasomal degradation. This process, termed "antibody-dependent intracellular neutralization," bridges adaptive and innate immune responses. Concurrently, TRIM21 activates inflammatory pathways (e.g., NF-κB, IRFs) to induce antiviral cytokines.
Clinically, TRIM21 is a major autoantigen in autoimmune diseases like Sjögren’s syndrome and systemic lupus erythematosus (SLE), where anti-TRIM21 autoantibodies serve as diagnostic markers. Its role in cancer is context-dependent, showing both tumor-suppressive and pro-oncogenic activities. TRIM21 antibodies are crucial tools for studying these mechanisms, enabling research into viral pathogenesis, autoimmune etiology, and therapeutic targeting of ubiquitination pathways.
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