| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | WRP; MEGAP; SRGAP2; ARHGAP14 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human SRGAP3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SRGAP3抗体的3篇示例参考文献(注：以下为虚构示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *SRGAP3 regulates dendritic spine morphogenesis and synaptic plasticity in cortical neurons*
**作者**: Smith A, et al.
**摘要**: 本研究利用特异性SRGAP3抗体，通过免疫荧光和Western blot技术，揭示了SRGAP3蛋白在小鼠皮层神经元树突棘形成中的关键作用。实验表明，SRGAP3缺失导致突触功能异常，提示其与神经发育障碍相关。
2. **文献名称**: *The role of SRGAP3 in autism spectrum disorder: Insights from human iPSC-derived neurons*
**作者**: Chen L, et al.
**摘要**: 通过在人诱导多能干细胞(iPSC)分化的神经元中结合SRGAP3抗体进行蛋白定位分析，研究发现自闭症患者来源的神经元中SRGAP3表达显著下调，并伴随突触传递异常，支持SRGAP3作为自闭症潜在生物标志物。
3. **文献名称**: *SRGAP3 interacts with Disrupted-in-Schizophrenia 1 (DISC1) to modulate neurodevelopment*
**作者**: Johnson R, et al.
**摘要**: 本文通过共免疫沉淀(Co-IP)和SRGAP3抗体验证，发现SRGAP3与精神分裂症相关蛋白DISC1存在直接相互作用，并共同调控神经前体细胞的迁移与分化，为精神疾病机制提供了新视角。
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建议通过PubMed或Google Scholar搜索以下关键词获取真实文献：
- **"SRGAP3 antibody" + "function"**
- **"SRGAP3" + "synaptic"**
- **"SRGAP3" + "neurodevelopmental disorders"**
SRGAP3 (SLIT-ROBO Rho GTPase-Activating Protein 3) is a gene located on chromosome 3p25.3. encoding a protein involved in regulating cytoskeletal dynamics and neuronal development. It belongs to the evolutionarily conserved SRGAP family, which interacts with ROBO receptors to modulate SLIT-ROBO signaling pathways. SRGAP3 plays critical roles in axonal guidance, dendritic morphogenesis, and neuronal migration during brain development. Dysregulation of SRGAP3 has been implicated in neurodevelopmental disorders, including autism spectrum disorders, intellectual disability, and schizophrenia, as well as certain cancers.
SRGAP3 antibodies are immunological tools designed to detect and quantify SRGAP3 protein expression in research applications. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study SRGAP3’s localization, expression patterns, and interactions in cellular and tissue contexts. Validating SRGAP3 antibodies is essential due to potential cross-reactivity with other SRGAP family members (e.g., SRGAP1/2/4) and splice variants. Researchers often employ knockout controls or siRNA-mediated knockdown to confirm specificity. Commercial SRGAP3 antibodies are typically raised against epitopes within the conserved RhoGAP domain or variable N-terminal regions. Understanding SRGAP3’s role in neuronal connectivity and disease pathogenesis continues to drive interest in developing reliable antibodies for mechanistic and diagnostic studies.
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