| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | spry-3 |
| WB Predicted band size | 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human SPRY3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SPRY3抗体的3篇示例参考文献(注：内容为虚构概括，仅供参考)：
1. **文献名称**：*SPRY3 Antibody-Based Detection of Tumor Suppressor Activity in Colorectal Cancer*
**作者**：Smith J, et al.
**摘要**：本研究利用SPRY3特异性抗体，通过免疫组化分析发现SPRY3在结直肠癌组织中表达显著下调，其低表达与患者预后不良相关，提示SPRY3可能作为抑癌基因通过调控MAPK信号通路抑制肿瘤进展。
2. **文献名称**：*Characterization of SPRY3 Polyclonal Antibody for Functional Studies in Neuronal Development*
**作者**：Lee H, et al.
**摘要**：文章报道了一种新型兔源SPRY3多克隆抗体的开发与验证，证实其可特异性识别内源性SPRY3蛋白。通过Western blot和免疫荧光实验，揭示了SPRY3在小鼠大脑皮层神经元迁移中的动态表达模式。
3. **文献名称**：*SPRY3 as a Biomarker in Pancreatic Ductal Adenocarcinoma: Validation by ELISA*
**作者**：Garcia R, et al.
**摘要**：研究采用SPRY3抗体建立ELISA检测方法，发现胰腺导管腺癌患者血清中SPRY3蛋白水平显著升高，且与肿瘤分期正相关，提示其可能作为液体活检标志物用于早期诊断。
SPRY3 (Sprouty RTK Signaling Antagonist 3) is a member of the Sprouty protein family, known for regulating receptor tyrosine kinase (RTK) signaling pathways, particularly the MAPK/ERK cascade, which influences cell proliferation, differentiation, and apoptosis. The SPRY3 gene, located on chromosome 17q21.2. encodes a cytoplasmic protein that modulates growth factor signaling by interacting with key pathway components. Research highlights its dual role in cancer: it may act as a tumor suppressor in certain contexts (e.g., colorectal and prostate cancers) through epigenetic silencing or downregulation, while exhibiting oncogenic properties in others (e.g., gliomas). Beyond oncology, SPRY3 is implicated in neurodevelopment, with links to autism spectrum disorders, and organogenesis, particularly in kidney and lung formation.
SPRY3 antibodies are essential tools for detecting and quantifying SPRY3 protein expression in research. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study tissue-specific localization, signaling interactions, and disease mechanisms. Commercial antibodies vary in host species (e.g., rabbit, mouse), clonality (monoclonal/polyclonal), and validation standards. Recent studies leverage these antibodies to explore SPRY3's potential as a therapeutic target or biomarker, particularly in cancers with dysregulated RTK pathways. However, inconsistent findings across cancer types underscore the need for context-specific functional analyses when interpreting SPRY3 antibody-based data.
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