| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HH17 |
| WB Predicted band size | 33 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于SPRY4抗体的模拟参考文献(示例格式，非真实文献)：
1. **《SPRY4 inhibits tumor progression in colorectal cancer via suppressing MAPK signaling》**
作者：Chen L, et al.
摘要：研究通过Western blot和免疫组化分析，发现SPRY4蛋白在结直肠癌组织中表达下调，其抗体检测显示低表达与患者预后不良相关。实验表明SPRY4通过抑制MAPK通路发挥抑癌作用。
2. **《Antibody-based profiling of SPRY4 in melanoma metastasis》**
作者：Wang Y, et al.
摘要：利用SPRY4特异性抗体进行组织芯片分析，发现SPRY4在转移性黑色素瘤中显著低表达，且其缺失促进细胞迁移。研究提示SPRY4抗体可作为潜在转移标志物检测工具。
3. **《SPRY4 antibody validation for embryonic developmental studies》**
作者：Smith JR, et al.
摘要：本文系统验证了多种SPRY4抗体的特异性，发现某克隆号抗体在小鼠胚胎肢芽发育模型中可精准检测SPRY4表达，证实其在调控FGF信号中的时空特异性作用。
注：以上为模拟示例，实际文献需通过PubMed/Google Scholar以"SPRY4 antibody"+"研究领域关键词"检索获取。建议结合具体研究方向筛选抗体应用类文献(如诊断标志物验证、信号机制研究等)。
SPRY4 antibody is a tool used to detect SPRY4 (Sprouty RTK Signaling Antagonist 4), a protein encoded by the *SPRY4* gene in humans. SPRY4 belongs to the Sprouty family of proteins, which are evolutionarily conserved regulators of receptor tyrosine kinase (RTK) signaling pathways, particularly the Ras/MAPK (mitogen-activated protein kinase) cascade. These proteins function as feedback inhibitors, modulating cellular responses to growth factors like FGF (fibroblast growth factor) and EGF (epidermal growth factor). SPRY4 is implicated in embryonic development, organogenesis (e.g., lung branching morphogenesis), and tissue homeostasis.
Dysregulation of SPRY4 has been linked to cancers, including colorectal, prostate, and lung cancers. Studies suggest SPRY4 acts as a tumor suppressor by antagonizing oncogenic signaling pathways (e.g., EGFR/RAS/MAPK). Reduced SPRY4 expression is associated with tumor progression, metastasis, and drug resistance. Additionally, *SPRY4* is a target of miR-21. an oncogenic microRNA, highlighting its role in cancer biology.
SPRY4 antibodies are widely used in research to study protein expression, localization, and function via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help elucidate SPRY4's role in cellular signaling, cancer mechanisms, and potential therapeutic targeting. Commercial SPRY4 antibodies are typically validated for specificity and sensitivity, with applications in both basic research and clinical biomarker studies.
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