| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FNRA; CD49e; VLA-5; VLA5A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ITGA5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ITGA5抗体的模拟参考文献示例(内容为学术场景模拟，非真实文献)：
1. **文献名称**：*Targeting ITGA5 with a functional antibody inhibits tumor metastasis in triple-negative breast cancer*
**作者**：Zhang L, et al.
**摘要**：研究证明抗ITGA5抗体通过阻断α5β1整合素与纤维连接蛋白的相互作用，抑制三阴性乳腺癌细胞的迁移和侵袭，并减少小鼠模型中肺转移。
2. **文献名称**：*ITGA5 blockade suppresses angiogenesis by disrupting endothelial cell adhesion and signaling*
**作者**：Mori S, et al.
**摘要**：抗ITGA5抗体通过干扰内皮细胞黏附及VEGF信号通路，显著抑制体内外血管生成，为抗肿瘤血管治疗提供新策略。
3. **文献名称**：*Anti-ITGA5 antibody attenuates fibrosis in experimental liver disease*
**作者**：Katsumi A, et al.
**摘要**：该研究利用ITGA5抗体抑制肝星状细胞活化，减少细胞外基质沉积，改善小鼠肝纤维化病理表型，提示其治疗纤维化疾病的潜力。
4. **文献名称**：*Mechanistic insights into ITGA5-mediated cell adhesion using a monoclonal antibody approach*
**作者**：Huang C, et al.
**摘要**：通过特异性抗体揭示ITGA5在细胞黏附中的构象变化及下游FAK/PI3K信号激活机制，为靶向整合素的药物设计提供理论基础。
(注：以上文献为示例性内容，实际引用需查询PubMed、Web of Science等数据库获取真实研究。)
The ITGA5 antibody targets integrin alpha-5 (ITGA5), a subunit that pairs with integrin beta-1 (ITGB1) to form the heterodimeric receptor α5β1. This receptor primarily binds fibronectin, a key extracellular matrix (ECM) protein, and mediates cell adhesion, migration, and signaling. ITGA5 is involved in processes like embryogenesis, angiogenesis, wound healing, and tumor progression, making it a focus in cancer research, fibrosis, and inflammatory diseases.
Antibodies against ITGA5 are widely used to detect its expression in tissues or cells via techniques like Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), or flow cytometry. They help study ITGA5's role in cell-ECM interactions, metastasis, or drug resistance. Some antibodies block ligand binding, enabling functional studies to inhibit integrin-mediated pathways.
Commercially available ITGA5 antibodies vary in clonality (monoclonal/polyclonal), species reactivity (human, mouse, rat), and epitope specificity (e.g., extracellular domain). Validation data, including knockout controls or application-specific citations, are critical for selecting reliable reagents. Suppliers like R&D Systems, Abcam, and Thermo Fisher offer antibodies validated for specific applications.
When using ITGA5 antibodies, consider sample preparation (native vs. denatured conditions) and fixation methods, as these affect epitope accessibility. Functional blocking assays may require native, conformation-sensitive antibodies. Proper controls (e.g., isotype-matched IgG) ensure specificity in experimental settings.
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