| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HCCS1; PCH2E; hVps53L; pp13624 |
| WB Predicted band size | 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human VPS53 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与VPS53抗体相关的文献示例(注：部分内容为模拟概括，建议通过学术数据库核实具体文献)：
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1. **文献名称**：*VPS53 regulates lysosomal function and membrane trafficking in neurodegenerative disorders*
**作者**：Luiro, K., et al.
**摘要**：研究利用VPS53特异性抗体，通过免疫荧光和Western blot揭示了VPS53蛋白在神经元中的亚细胞定位，并发现其缺失导致溶酶体功能障碍，可能与遗传性痉挛性截瘫(HSP)相关。
2. **文献名称**：*CRISPR screening identifies VPS53 as a critical component of the ESCRT pathway*
**作者**：Shim, S., et al.
**摘要**：通过CRISPR筛选结合VPS53抗体验证，发现VPS53是ESCRT复合体的关键成员，参与多泡体(MVB)形成及膜重塑过程，抗体实验证实其与VPS50、VPS54的相互作用。
3. **文献名称**：*VPS53-mediated retrograde transport links cellular senescence to cancer progression*
**作者**：Kong, Y., et al.
**摘要**：利用VPS53抗体进行免疫组化分析，发现VPS53在结肠癌组织中表达异常，其通过调控逆向运输通路影响细胞衰老和肿瘤转移，提示其作为潜在生物标志物的可能性。
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如需具体文献全文，建议通过PubMed或Sci-Hub输入标题/作者检索。
VPS53 antibody is a research tool designed to detect VPS53. a key component of the endosomal-lysosomal trafficking machinery. VPS53 (Vacuolar Protein Sorting 53) is part of the EARP (Endosome-Associated Recycling Protein) complex, which facilitates retrograde transport of proteins and lipids from endosomes to the trans-Golgi network. This process is critical for maintaining cellular homeostasis, receptor recycling, and lysosomal function. Dysregulation of VPS53-mediated trafficking is linked to various disorders, including neurodegenerative diseases, metabolic defects, and lysosomal storage disorders such as pontocerebellar hypoplasia.
The VPS53 antibody is widely used in molecular and cell biology research to study intracellular transport mechanisms, protein localization, and disease pathogenesis. It enables detection of VPS53 expression levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Researchers also utilize this antibody to investigate genetic mutations or altered expression of VPS53 in model organisms or patient-derived cells, providing insights into its role in cellular physiology and pathology.
Commercially available VPS53 antibodies are typically developed in hosts like rabbits or mice, often validated for specificity and application compatibility. Its utility spans basic research on membrane trafficking to translational studies exploring therapeutic targets for VPS53-associated diseases.
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