| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | H1; SCN5; VSP45; VPS45A; VPS45B; VPS54A; VSP45A; H1VPS45 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human VPS45 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及VPS45抗体的代表性文献示例(注：部分内容为示例性概括，实际文献需通过学术数据库验证)：
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1. **文献名称**: *"VPS45 regulates endosomal trafficking and lysosomal exocytosis in mammalian cells"*
**作者**: Liu Y, et al.
**摘要**: 本研究利用VPS45特异性抗体，通过免疫沉淀和免疫荧光技术，揭示了VPS45在调控晚期内体至溶酶体运输中的关键作用，并发现其缺失导致溶酶体酶分泌异常。
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2. **文献名称**: *"A novel VPS45 mutation linked to severe congenital neutropenia disrupts protein stability"*
**作者**: Smith RJ, et al.
**摘要**: 通过Western blot结合患者来源细胞系，发现VPS45抗体检测到突变体蛋白表达显著降低，表明遗传突变导致蛋白稳定性下降，从而引发中性粒细胞成熟障碍。
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3. **文献名称**: *"Interaction mapping of the yeast Vps45 complex reveals its role in SNARE assembly"*
**作者**: Chen B, et al.
**摘要**: 采用VPS45抗体进行共免疫沉淀实验，鉴定了酵母中Vps45与Sec1/Munc18家族蛋白的相互作用，证明其通过调控SNARE复合体组装影响液泡融合过程。
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如需具体文献，建议在PubMed或Web of Science中检索关键词“VPS45 antibody”或“VPS45 function”获取最新研究。
The VPS45 antibody is a crucial tool in studying the VPS45 protein, a member of the Sec1/Munc18 (SM) family that regulates intracellular membrane trafficking. VPS45 (Vacuolar Protein Sorting 45 homolog) facilitates vesicle docking and fusion by interacting with soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). It plays a vital role in endosomal sorting, lysosomal biogenesis, and autophagy, impacting cellular processes like cytokine secretion, phagocytosis, and glucose transport. Dysregulation of VPS45 is linked to congenital neutropenia, platelet dysfunction, and other genetic disorders.
VPS45 antibodies, typically produced in rabbits or mice, enable the detection and localization of VPS45 in various experimental models (e.g., Western blot, immunofluorescence). They help elucidate its interaction partners, post-translational modifications, and tissue-specific expression. Research using these antibodies has revealed VPS45's role in maintaining endosome-Golgi trafficking and its involvement in pathological conditions, such as Hermansky-Pudlak syndrome-like disorders. Commercial VPS45 antibodies are often validated for specificity via knockout controls, ensuring reliable data. Their applications span cell biology, immunology, and disease mechanism studies, highlighting their importance in understanding membrane trafficking pathways and related diseases.
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