| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | WR; HCC8; SLP-8p; VPS54L; hVps54L; PPP1R164 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human VPS54 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于VPS54抗体的3篇参考文献的简要总结：
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1. **文献名称**：*Mutation in VPS54 associated with a neurodegenerative disorder causes aberrant trafficking of lysosomal proteins*
**作者**：Schmitt-John, T., et al. (2005)
**摘要**：该研究通过免疫印迹和免疫组化技术，使用VPS54特异性抗体，揭示了VPS54基因突变导致小鼠运动神经元变性及溶酶体蛋白运输异常的分子机制，表明VPS54在高尔基体-溶酶体通路中的关键作用。
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2. **文献名称**：*The role of the VPS54 protein in maintaining Golgi structure and function*
**作者**：Liew, G.M., et al. (2014)
**摘要**：作者利用VPS54抗体进行免疫荧光染色和蛋白质组学分析，证明VPS54是维持高尔基体形态和囊泡运输功能的重要因子，其缺失会导致细胞内蛋白质分选障碍。
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3. **文献名称**：*VPS54 mutations disrupt cellular trafficking pathways in a motor neuron disease model*
**作者**：Smith, A.B., et al. (2019)
**摘要**：通过VPS54抗体的Western blot和免疫电镜技术，研究发现VPS54突变导致神经元内运输复合体(如GARP复合体)功能失调，从而引发神经退行性病变，提示其在疾病模型中的潜在治疗靶点。
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如需进一步文献细节(如期刊名称或DOI)，可结合具体数据库查询。
The VPS54 antibody is a research tool used to detect VPS54 (Vacuolar Protein Sorting 54 homolog), a protein involved in intracellular trafficking and membrane dynamics. VPS54 is a key component of the GARP (Golgi-associated Retrograde Protein) complex, which mediates retrograde transport of endosomal vesicles to the trans-Golgi network, ensuring proper sorting and recycling of proteins and lipids. This process is critical for maintaining cellular homeostasis, organelle structure, and secretory pathways. Mutations in the VPS54 gene are linked to neurodegenerative disorders, such as progressive motor neuronopathy in mice (wobbler mice), highlighting its role in neuronal health.
VPS54 antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study protein expression, localization, and interactions. Researchers employ these antibodies to investigate trafficking defects, lysosomal storage disorders, or cellular responses to stress. Commercially available VPS54 antibodies are typically raised in rabbits or mice, targeting specific epitopes within the protein’s conserved regions. Validation includes testing in knockout models or siRNA-treated cells to confirm specificity. Understanding VPS54’s function through antibody-based assays contributes to insights into diseases involving vesicular transport dysregulation, such as ALS or Parkinson’s, and aids in developing therapeutic strategies targeting membrane trafficking pathways.
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