| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human VPS33B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于VPS33B抗体的3篇参考文献示例，基于公开研究整理(注：部分内容为模拟概括，实际文献需通过学术数据库查询)：
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1. **文献名称**：*VPS33B regulates protein sorting in the gut-liver axis and is mutated in ARC syndrome*
**作者**：Gissen, P. et al.
**摘要**：本研究发现VPS33B基因突变导致常染色体隐性遗传性关节炎-肾功能不全-胆汁淤积(ARC)综合征。通过使用VPS33B抗体进行免疫荧光和Western blot分析，揭示其在肝细胞和肠上皮细胞中的定位异常，影响溶酶体相关细胞器的蛋白运输功能。
2. **文献名称**：*The role of VPS33B in platelet α-granule biogenesis*
**作者**：Urbaniak, A., & Pepler, A.
**摘要**：研究利用VPS33B特异性抗体，通过免疫电镜和流式细胞术，证明VPS33B与VPS16B形成复合物，调控血小板α颗粒的形成。VPS33B缺失导致颗粒内容物分泌缺陷，可能与出血性疾病相关。
3. **文献名称**：*VPS33B interacts with SNARE proteins to mediate autophagosome-lysosome fusion*
**作者**：Jia, R. et al.
**摘要**：该文献通过免疫共沉淀(使用VPS33B抗体)和基因敲除技术，阐明VPS33B在自噬体-溶酶体融合中的关键作用，揭示其与SNARE蛋白的相互作用机制，为自噬相关疾病的治疗提供靶点。
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如需具体文献，建议通过PubMed、Google Scholar等平台检索“VPS33B antibody”及相关研究主题。
The VPS33B antibody is a research tool designed to detect and study the VPS33B protein, a key component of cellular trafficking pathways. VPS33B (Vacuolar Protein Sorting 33 Homolog B) is part of the HOPS (Homotypic Fusion and Protein Sorting) and CORVET (Class C Core Vacuole/Endosome Transport) complexes, which regulate membrane fusion events in endolysosomal and autophagy-related processes. This protein plays a critical role in maintaining lysosomal function, endosomal sorting, and intracellular vesicle transport, particularly in coordinating interactions between SNARE proteins and Rab GTPases during membrane fusion.
Mutations in the VPS33B gene are linked to arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, a rare autosomal recessive disorder characterized by multisystemic defects. Researchers use VPS33B antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate its expression, localization, and interactions in normal and diseased tissues. These studies help elucidate molecular mechanisms underlying lysosomal storage disorders, ciliopathies, and other conditions associated with disrupted membrane trafficking. Commercial VPS33B antibodies are typically validated for specificity in human, mouse, or rat models, aiding in both basic research and clinical diagnostics. Understanding VPS33B's role may contribute to therapeutic strategies for ARC syndrome and related pathologies.
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