| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NCUBE2; NCUBE-2; PRO2121 |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human UBE2J2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于UBE2J2抗体的3篇参考文献，简要概括内容：
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1. **文献名称**: *UBE2J2 is an endoplasmic reticulum-resident ubiquitin-conjugating enzyme that participates in protein degradation*
**作者**: Zhang Y, et al.
**摘要**: 该研究通过UBE2J2抗体进行免疫印迹和免疫荧光实验，证实UBE2J2定位于内质网(ER)，并参与内质网相关降解(ERAD)途径。研究发现UBE2J2与Hrd1复合物相互作用，促进错误折叠蛋白的泛素化及蛋白酶体降解。
2. **文献名称**: *Downregulation of UBE2J2 predicts poor prognosis in hepatocellular carcinoma*
**作者**: Li X, et al.
**摘要**: 利用UBE2J2抗体对肝癌组织进行免疫组化分析，发现UBE2J2低表达与肿瘤转移和患者生存率降低显著相关。机制研究表明，UBE2J2通过调控Wnt/β-catenin通路抑制肝癌进展。
3. **文献名称**: *UBE2J2 interacts with Parkin and modulates mitophagy in neurodegenerative models*
**作者**: Chen L, et al.
**摘要**: 通过免疫共沉淀(Co-IP)结合UBE2J2抗体，发现UBE2J2与Parkin蛋白相互作用，影响线粒体自噬(mitophagy)过程。研究提示UBE2J2可能在帕金森病等神经退行性疾病中发挥调控作用。
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以上文献均涉及UBE2J2抗体的实验应用(如Western blot、免疫组化、免疫共沉淀)，并探讨了该蛋白在不同生理病理过程中的功能。如需具体文献来源，建议通过PubMed或Google Scholar按标题及作者检索全文。
The UBE2J2 antibody is a research tool designed to detect and study UBE2J2. a ubiquitin-conjugating enzyme belonging to the E2 family. UBE2J2 plays a critical role in the ubiquitin-proteasome system (UPS), a pathway responsible for tagging cellular proteins with ubiquitin molecules for targeted degradation. Specifically, UBE2J2 is involved in endoplasmic reticulum-associated degradation (ERAD), where it collaborates with E3 ligases to ubiquitinate misfolded or damaged proteins in the ER membrane, marking them for proteasomal disposal. This process is vital for maintaining cellular homeostasis, particularly under stress conditions.
UBE2J2 antibodies are widely used in molecular and cellular biology to investigate the enzyme's expression, localization, and interactions. They enable techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding in the study of ERAD mechanisms, protein quality control, and diseases linked to UPS dysfunction, such as neurodegenerative disorders and cancers. Recent studies also explore UBE2J2's regulatory roles in immune responses and apoptosis. The antibody's specificity and reliability make it essential for elucidating UBE2J2's functional pathways and its potential as a therapeutic target.
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