| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CPSF5; CFIM25 |
| WB Predicted band size | 26 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NUDT21抗体的参考文献示例(注：文献为虚构示例，实际引用需根据真实文献调整):
1. **文献名称**: "NUDT21 Regulates Alternative Polyadenylation in Glioblastoma"
**作者**: Smith A, et al.
**摘要**: 本研究利用NUDT21特异性抗体进行免疫沉淀和Western blot分析，揭示其在胶质母细胞瘤中通过调控mRNA 3'端多聚腺苷酸化影响肿瘤细胞增殖的机制。
2. **文献名称**: "CRISPR Screening Identifies NUDT21 as a Cell Cycle Checkpoint Regulator"
**作者**: Johnson B, et al.
**摘要**: 通过NUDT21抗体检测敲低后的蛋白表达，结合功能实验证明其通过结合CCNB1 mRNA影响细胞周期G2/M期转换。
3. **文献名称**: "NUDT21 Antibody Validation for Clinical Biomarker Analysis"
**作者**: Lee C, et al.
**摘要**: 系统性验证NUDT21抗体的特异性(包括敲除/过表达细胞系)，并应用于乳腺癌组织芯片，证实其表达与患者生存率显著相关。
4. **文献名称**: "NUDT21 Interaction Network Revealed by IP-MS"
**作者**: Garcia D, et al.
**摘要**: 使用NUDT21抗体进行免疫沉淀-质谱联用分析，鉴定出与CPSF复合物的相互作用，阐明其在pre-mRNA加工中的核心作用。
建议通过PubMed或Google Scholar以关键词"NUDT21 antibody"+"validation/application"检索最新文献，并优先选择经过抗体验证(如KO验证、应用实验数据)的研究。
The NUDT21 antibody is a tool used to detect NUDT21 (Nudix Hydrolase 21), also known as CFIm25. a key subunit of the cleavage factor Im (CFIm) complex involved in pre-mRNA 3'-end processing. NUDT21 plays a critical role in mRNA polyadenylation, regulating alternative polyadenylation (APA) sites to influence transcript diversity, stability, and protein expression. Dysregulation of NUDT21 has been linked to cancers, neurological disorders, and viral infections.
NUDT21 antibodies are widely employed in molecular biology research to study its expression, localization, and interactions. They are used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore NUDT21's role in APA-mediated gene regulation, cellular differentiation, and disease mechanisms. For instance, reduced NUDT21 levels correlate with shortened 3' untranslated regions (3'UTRs) in cancer-related genes, promoting oncogenesis. These antibodies also aid in identifying NUDT21's involvement in neurodevelopment and responses to cellular stress.
Commercially available NUDT21 antibodies are typically raised in rabbits or mice against specific epitopes, validated for specificity and sensitivity. Researchers rely on them to dissect post-transcriptional regulatory networks and potential therapeutic targets. Their applications extend to clinical studies, where altered NUDT21 expression serves as a biomarker for disease prognosis or drug response evaluation.
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