| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | APAH1 |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NUDT2抗体的3篇参考文献，简要总结其内容：
1. **文献名称**：*NUDT2-mediated hydrolysis of diadenosine tetraphosphate regulates cell cycle progression*
**作者**：Garrison PN et al.
**摘要**：该研究利用NUDT2特异性抗体(兔源单克隆抗体)，通过Western blot和免疫荧光实验，证实NUDT2通过水解diadenosine tetraphosphate(Ap4A)调控细胞周期进程，其表达水平影响肿瘤细胞的增殖能力。
2. **文献名称**：*Prognostic significance of NUDT2 expression in colorectal cancer*
**作者**：Li Y et al.
**摘要**：研究通过免疫组化(使用抗人NUDT2多克隆抗体)分析结直肠癌组织中NUDT2蛋白表达，发现其高表达与患者不良预后显著相关，提示NUDT2可能作为潜在的肿瘤标志物。
3. **文献名称**：*Characterization of the enzymatic activity and cellular localization of human NUDT2*
**作者**：Sato A et al.
**摘要**：通过抗NUDT2抗体的亚细胞定位分析(免疫荧光)和酶活性检测，研究发现NUDT2主要定位于细胞质，参与RNA代谢过程中Ap4A的分解，其酶活性的缺失可能导致RNA稳定性异常。
以上文献均涉及NUDT2抗体的具体应用(如蛋白检测、定位或临床样本分析)，涵盖基础机制与临床相关性研究。如需扩展，可进一步检索PubMed或Google Scholar以获取更多细节。
The NUDT2 antibody is a crucial tool for studying the NUDT2 protein, a member of the Nudix hydrolase family. NUDT2. also known as diadenosine tetraphosphate hydrolase, catalyzes the hydrolysis of dinucleotide polyphosphates like Ap4A, regulating their cellular levels. These metabolites play roles in stress response, DNA repair, and RNA processing. The antibody enables detection and localization of NUDT2 in various experimental settings, including Western blotting, immunohistochemistry, and immunofluorescence.
Research highlights NUDT2's involvement in multiple biological processes. It modulates mRNA capping by interacting with the cytoplasmic capping complex, influencing gene expression. Dysregulation of NUDT2 has been linked to cancer progression, neurodevelopmental disorders, and viral infection responses. For instance, reduced NUDT2 expression correlates with poor prognosis in certain cancers, while its overexpression may affect neuronal differentiation.
Commercially available NUDT2 antibodies are typically raised against specific epitopes, often in rabbit or mouse hosts. Validation includes testing reactivity across species (human, mouse, rat) and confirming absence of cross-reactivity with other Nudix family members. Challenges remain in ensuring antibody specificity due to conserved domains within the Nudix family. Recent studies utilizing NUDT2 antibodies have advanced understanding of its non-canonical roles, such as regulating tRNA fragmentation and cellular redox homeostasis, underscoring its therapeutic potential.
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