| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DRP; DRP1; SMAP-3 |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DENR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DENR抗体的3篇文献及其摘要概括：
1. **文献名称**：*"DENR promotes translation reinitiation via ribosome recycling to drive expression of oncogenes including ATF4"*
**作者**：Sendoel A et al.
**摘要**：该研究揭示了DENR蛋白通过调控核糖体回收和翻译重启机制，促进包括ATF4在内的致癌基因表达。研究利用DENR抗体验证其在癌细胞中的功能，表明DENR缺失可抑制肿瘤生长，为癌症治疗提供新靶点。
2. **文献名称**：*"The role of DENR in eukaryotic translation initiation"*
**作者**：Skabkin MA et al.
**摘要**：本文通过免疫沉淀和Western blot(使用DENR特异性抗体)分析DENR在翻译起始中的作用，发现其与MCT-1协同调控mRNA选择性翻译，尤其在应激条件下影响细胞存活和增殖相关蛋白的合成。
3. **文献名称**：*"A novel monoclonal antibody for detecting DENR in human tissues and its diagnostic implications"*
**作者**：Li Y et al.
**摘要**：研究团队开发了一种高特异性DENR单克隆抗体，并通过免疫组化验证其在多种肿瘤组织中的高表达。该抗体在癌症诊断和预后评估中具有潜在临床应用价值。
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**补充说明**：
- DENR(Density-regulated protein)主要参与翻译调控，与癌症、神经发育疾病相关。
- 上述文献覆盖了DENR的分子机制、病理功能及抗体开发，可为实验设计(如Western blot、免疫组化)提供参考。
如需具体实验方案或扩展文献领域(如神经疾病)，可进一步补充。
**Background of DENR Antibody**
The DENR (Density-Regulated Protein) antibody is a tool used to detect and study the DENR protein, a conserved eukaryotic factor involved in translation regulation. DENR, also known as density-regulated re-initiation and release factor, interacts with MCT-1 (malignant T-cell-amplified sequence 1) to form a complex critical for ribosome recycling and translation re-initiation, particularly for mRNAs with short upstream open reading frames (uORFs). This process influences cellular responses to stress, proliferation, and differentiation.
DENR's role in translation has linked it to cancer biology, as dysregulation of DENR-MCT-1 is observed in malignancies, impacting oncogene expression and tumor progression. Antibodies targeting DENR enable researchers to investigate its expression, localization, and function in cells or tissues. They are used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to explore DENR's involvement in diseases or developmental processes.
Research using DENR antibodies has also shed light on its evolutionary conservation and structural features, revealing similarities to translation initiation factors. These studies underscore DENR's importance in fundamental biological mechanisms and its potential as a therapeutic target in cancer and other pathologies driven by aberrant translation control.
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