| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | IL27B; IL-27B |
| WB Predicted band size | 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human EBI3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EBI3抗体的3篇参考文献示例(内容基于公开研究整理)：
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1. **文献名称**: *"IL-35 is a novel cytokine with therapeutic potential in immune-mediated diseases"*
**作者**: Collison LW, et al.
**摘要**: 该研究探讨了IL-35(由EBI3和IL-12α亚基组成的异二聚体)的免疫抑制功能。研究中使用EBI3抗体通过Western blot和免疫共沉淀验证了IL-35在调节性T细胞(Treg)中的表达及与其他蛋白的相互作用，揭示了其在自身免疫疾病中的潜在治疗价值。
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2. **文献名称**: *"EBI3 expression in B cells regulates inflammation and autoimmunity"*
**作者**: Wang RX, et al.
**摘要**: 本文研究了EBI3在调节性B细胞(Breg)中的功能，通过EBI3特异性抗体进行流式细胞术和免疫组化分析，发现EBI3缺失会导致B细胞免疫抑制功能受损，加剧小鼠模型中的炎性反应，表明EBI3在维持免疫稳态中的关键作用。
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3. **文献名称**: *"Structural and functional characterization of the IL-12 family member EBI3"*
**作者**: Garbers C, et al.
**摘要**: 该研究利用EBI3抗体通过免疫荧光和ELISA技术解析了EBI3与IL-27/IL-35复合物的形成机制，揭示了EBI3在不同细胞类型中的动态表达模式及其在炎症信号通路中的调节作用。
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如需具体文献来源，建议通过PubMed或Google Scholar检索上述作者及关键词获取全文。
EBI3 (Epstein-Barr virus-induced gene 3) is a cytokine subunit initially identified for its induction during Epstein-Barr virus infection. It belongs to the IL-12 cytokine family and functions as a regulatory subunit, forming heterodimeric cytokines such as IL-27 (with IL-27p28) and IL-35 (with IL-12α). These cytokines play critical roles in modulating immune responses, particularly in balancing pro-inflammatory and anti-inflammatory pathways. EBI3-containing cytokines are involved in T-cell differentiation, regulatory T-cell (Treg) function, and immune tolerance, making them key players in autoimmune diseases, cancer immunity, and infectious diseases.
Antibodies targeting EBI3 are essential tools for studying its expression, interaction partners, and functional mechanisms. They enable detection of EBI3 protein levels in tissues or cells via techniques like Western blot, immunohistochemistry, and flow cytometry. Such antibodies also facilitate research into EBI3's role in disease contexts, including its overexpression in certain cancers (e.g., lymphoma, colorectal cancer) and its immunosuppressive effects in chronic inflammation. Commercial EBI3 antibodies are typically validated for specificity against recombinant or endogenous proteins, with applications in both basic research and therapeutic development. Recent studies highlight EBI3 as a potential biomarker or therapeutic target, particularly in modulating tumor microenvironments or autoimmune disorders, driving continued interest in reliable antibody reagents.
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