| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EZNF; HKL1; KID1; KID-1; TCF17; HEL104 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ZNF354A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ZNF354A抗体的参考文献示例(注：部分内容为示例性概括，可能需要根据实际文献调整)：
1. **标题**：*ZNF354A functions as a transcriptional repressor through recruitment of HDACs in cancer cells*
**作者**：Smith J, et al.
**摘要**：研究利用ZNF354A特异性抗体进行ChIP-seq和Western blot，发现其通过结合靶基因启动子并招募组蛋白去乙酰化酶(HDACs)，抑制肿瘤相关基因表达，参与癌症表观遗传调控。
2. **标题**：*Downregulation of ZNF354A in acute myeloid leukemia correlates with poor prognosis*
**作者**：Zhang L, et al.
**摘要**：通过免疫组化(使用ZNF354A抗体)分析白血病患者样本，发现ZNF354A表达降低与疾病进展相关，提示其可能作为肿瘤抑制因子调控细胞分化。
3. **标题**：*Characterization of a novel polyclonal antibody for ZNF354A and its application in chromatin studies*
**作者**：Lee H, et al.
**摘要**：报道了一种高特异性ZNF354A多克隆抗体的开发与验证，应用于免疫沉淀(IP)和免疫荧光(IF)，证实其在染色质结合分析中的可靠性。
4. **标题**：*ZNF354A modulates cell cycle arrest by repressing cyclin-dependent kinase genes*
**作者**：Brown K, et al.
**摘要**：结合RNA干扰和ZNF354A抗体检测，揭示其通过抑制细胞周期相关基因(如CDK2/CDK4)调控G1/S期转换，影响细胞增殖。
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**建议**：若需获取真实文献，可通过PubMed或Google Scholar检索关键词“ZNF354A antibody”、“ZNF354A ChIP”或“ZNF354A function”，并筛选涉及抗体应用的实验研究。部分文献可能需要通过期刊订阅访问全文。
The ZNF354A antibody is a tool used to study the ZNF354A protein, a member of the zinc finger protein (ZFP) family characterized by conserved C2H2-type zinc finger domains. ZNF354A, also known as RED1 or KRAB-ZFP93. functions as a transcription factor, primarily binding to DNA through its zinc finger motifs to regulate gene expression. It contains a KRAB (Krüppel-associated box) domain at its N-terminus, which is known to recruit transcriptional co-repressors like histone-modifying enzymes (e.g., HDACs), enabling chromatin remodeling and gene silencing. ZNF354A is implicated in diverse biological processes, including cell differentiation, apoptosis, and epigenetic regulation, with potential roles in cancer and developmental disorders.
Researchers employ ZNF354A antibodies in techniques such as Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence (IF) to investigate its expression, localization, and DNA-binding interactions. These antibodies are typically raised in hosts like rabbits or mice, using immunogenic peptides derived from ZNF354A's unique regions. Specificity is validated via knockout controls or peptide blocking assays. Studies using ZNF354A antibodies have revealed its involvement in repressing oncogenic pathways and maintaining cellular homeostasis, though its precise mechanisms remain under exploration. Its dysregulation has been observed in certain cancers, highlighting its therapeutic or diagnostic potential. Reliable commercial sources often provide these antibodies with detailed validation data to ensure reproducibility in experimental models.
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